Thermo Scientific™ TMT10plex™ Isobaric Label Reagents and Kits

Description

• TMT10plex Isobaric Label Reagents and Kits contain ten isobaric compounds with the same mass and chemical structure (i.e., isotopomeric) composed of an amine-reactive NHS-ester group, a spacer arm, and a mass reporter
• Up to ten different peptide samples prepared from cells or tissues can be labeled in parallel and then combined for analysis
• For each sample, a unique reporter mass (i.e., TMT10 126-131 Da) in the low-mass region of the high-resolution MS/MS spectrum is used to measure relative protein expression levels during peptide fragmentation and tandem mass spectrometry
• The Tandem Mass Tag (TMT) Isobaric Label reagents and kits are specifically designed to enable identification and quantitation of proteins in different samples using tandem mass spectrometry
• The TMT10plex label reagents share an identical structure with TMTzero, TMTduplex, and TMTsixplex reagents, but contain different numbers and combinations of 13C and 15N isotopes in the mass reporter
• The different isotopes result in a 10-plex set of tags that have mass differences in the reporter that can be detected using high resolution Orbitrap MS instruments
• Advantages of the TMT10plex label reagents include increased multiplex relative quantitation, increased sample throughput, and fewer missing quantitative values among samples
• TMT10plex label reagents are ideal for analysis of multiple protein samples from inhibitor dose response experiments, time course experiments, or improving statistics on biological replicates
• Features of TMT10plex Isobaric Label reagent sets and mass tagging kits • Powerful —multiplexed MS analysis increases sample throughput and enables relative quantitation of up to ten different samples derived from cells, tissues, or biological fluids • Consistent —identical reagent structure and performance among TMTzero, TMTduplex, TMTsixplex, and TMT10plex reagents allows efficient transition from method development to multiplex quantitation, enabling biomarker discovery research across platforms and datasets • Robust —increased multiplex capability results in fewer missing quantitative values among samples and higher confidence among replicates • Efficient —amine-reactive, NHS-ester-activated reagents ensure efficient labeling of all peptides regardless of protein sequence or proteolytic enzyme specificity • Compatible —optimized for use with high resolution Orbitrap MS/MS platforms, with data analysis fully supported by Proteome Discoverer 2.3
• Applications: • Protein identification and quantitation from multiple samples of cells, tissue, or biological fluids • Protein expression profiling of normal vs
• disease states or control vs
• treated • Quantitative analysis of proteins for which no antibodies are available • Identification and quantitation of membrane and post-translationally modified proteins • Identification and quantification of hundreds to thousands of proteins in a single experiment TMT Reagents are provided as standalone sets, a 96-well format, or in optimized kit formats containing all necessary reagents and controls for maximum flexibility, convenience, and reliability
• When combined with the industry-leading, high resolution Thermo Scientific Orbitrap instruments and software, TMT10plex reagents provide integrated total solutions for quantitative protein expression analysis.