Molecular Probes™ Pacific Orange™ and Pacific Blue™ Antibody Labeling Kits

Description

• Pacific Orange and Pacific Blue antibody labeling kits enable attachment of a violet light-excitable fluorescent label on antibodies (up to 100 μg per reaction)
• Each kit contains enough dye for five reactions
• A primary antibody directly labeled with a fluorophore often produces lower background fluorescence and less nonspecific binding
• Primary antibodies of the same isotype or derived from the same species can be multiplexed if they are directly labeled with compatible fluorophores
• Using Pacific Blue and Pacific Orange dyes simultaneously allows you to excite both dyes at the same wavelength while their emissions are differentially distinguished
• Pacific Orange and Pacific Blue antibody labeling kits allow the labeling of small amounts of antibody with a violet light-excitable dye (up to 100 μg of antibody per reaction)
• Small amounts of other proteins (>40 kDa) can also be labeled using these kits
• Features of these antibody labeling kits • Pacific Blue excitation and emission maxima: 410/455 nm • Pacific Orange excitation and emission maxima: 405/551 nm • Labeled proteins typically ready to use in 90 min
• (∼15 min
• hands-on time) • Optimized for small-scale labeling of any protein >40 kDa • Stabilizing proteins should be removed from the sample before labeling • Purified using convenient spin filters • Includes detailed instructions for determining degree of labeling (DOL) These kits contain everything you need to perform five separate labeling reactions as well as to purify the resulting conjugates
• Conjugates are ideal for multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISA, immunocytochemistry, indirect FISH, and more
• We are ISO 13485:2000 certified.