Thermo Scientific™ EZ-Link™ NHS-PEG4-Biotin

Description

• Thermo Scientific™ EZ-Link NHS-PEG 4 -Biotin is a pegylated, water-soluble reagent for simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing macromolecules
• Protein labeling - biotinylate antibodies or other proteins for detection or purification using streptavidin probes or resins Amine-reactive - reacts with primary amines (-NH2), such as the side-chain of lysines (K) or the amino-termini of polypeptides Pegylated - spacer arm contains a hydrophilic, 4-unit, polyethylene glycol (PEG) group Enhances solubility - pegylation imparts water solubility to the biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution Irreversible - forms permanent amide bonds; spacer arm cannot be cleaved Long reach - spacer arm (total length added to target) is 29 angstroms; this reduces steric hindrance when binding to avidin molecules