Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin

Description

• Thermo Scientific™ EZ-Link Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines
• Protein labeling - biotinylate antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes Cell surface labeling - biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes Amine-reactive - reacts with primary amines (-NH2), such as lysine side-chains, or the amino-termini of polypeptides Soluble - charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds Irreversible - forms permanent amide bonds; spacer arm cannot be cleaved Medium length - spacer arm (total length added to target) is 22.4 angstroms; provides excellent balance between adequate length (minimal steric hindrance for biotin binding) and modest mass