PI21450
Thermo Scientific™ EZ-Link™ NHS-PEG Solid-Phase Biotinylation Kit - Mini-Spin Columns
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI21450-Each-of-1 | In Stock | ₹ 52,448.28 |
PI21450 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 52,448.28
GST (18%): ₹ 9,440.69
Total Price: ₹ 61,888.97
Product Type
NHS-PEG Solid-Phase Biotinylation Kit
Content And Storage
Sufficient For: 8 labeling reactions, each with 0.1 to 1 mg of antibody• Ni-NTA Spin Columns, 0.2 mL, 8 columns• PBS pack (makes 500 mL), 1 pack• NHS-PEG4-Biotin, 8 x 2 mg• Imidazole Stock Solution (4M), 5 mL• Collection Tubes (2mL), 30 tubesStore at 4°C.
Product Line
EZ-Link™
Spacer
Pegylated
Quantity
8 reactions
Labeling Scale
0.5 to 1 mg
Label or Dye
Biotin
Labeling Target
IgG
Related Products
Description
- This innovative antibody-labeling system uses nickel-chelated agarose to temporarily immobilize antibody molecules via their histidine-rich Fc regions
- Once held in place on the resin, the antibody can be biotinylated at either sulfhydryl groups (after mild reduction or disulfide bonds) or primary amines
- Excess labeling reagent and byproducts are then washed away before recovering the labeled and purified antibody from the resin using a mild imidazole solution
- No gel filtration or dialysis is needed
- Four kits are available for small (0.1-1mg) or large (1-10mg) antibody samples using either amine-directed (NHS ester) or sulfhydryl-directed (maleimide) labeling reagents
- Highlights: Fast labeling and purification - the entire procedure takes only one hour (two hours for sulfhydryl labeling kits) Easy removal of spent and excess labeling reagent - simply wash away the reaction byproducts - no need for dialysis or gel filtration No dilution effects - solid-phase method allows initially dilute antibodies to be recovered in a smaller volume after labeling Optimized protocols - specific protocols for antibody ensure appropriate level of labeling (2-5 biotins per antibody molecule), minimizing possibility of inactivation caused by overlabeling Sufficient reagents for eight biotin-labeling experiments - Thermo Scientific No-Weigh Packaging ensures that the biotin reagent is fully active for eight separate experiments
Compare Similar Items
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Product Type: NHS-PEG Solid-Phase Biotinylation Kit
Content And Storage: Sufficient For: 8 labeling reactions, each with 0.1 to 1 mg of antibody• Ni-NTA Spin Columns, 0.2 mL, 8 columns• PBS pack (makes 500 mL), 1 pack• NHS-PEG4-Biotin, 8 x 2 mg• Imidazole Stock Solution (4M), 5 mL• Collection Tubes (2mL), 30 tubesStore at 4°C.
Product Line: EZ-Link™
Spacer: Pegylated
Quantity: 8 reactions
Labeling Scale: 0.5 to 1 mg
Label or Dye: Biotin
Labeling Target: IgG
Product Type:
NHS-PEG Solid-Phase Biotinylation Kit
Content And Storage:
Sufficient For: 8 labeling reactions, each with 0.1 to 1 mg of antibody• Ni-NTA Spin Columns, 0.2 mL, 8 columns• PBS pack (makes 500 mL), 1 pack• NHS-PEG4-Biotin, 8 x 2 mg• Imidazole Stock Solution (4M), 5 mL• Collection Tubes (2mL), 30 tubesStore at 4°C.
Product Line:
EZ-Link™
Spacer:
Pegylated
Quantity:
8 reactions
Labeling Scale:
0.5 to 1 mg
Label or Dye:
Biotin
Labeling Target:
IgG
Product Type: NHS-PEG4 Biotinylation Kit
Content And Storage: Sufficient For: 8 labeling reactions, each with 1 to 10 mg of antibody• EZ-Link NHS-PEG4-Biotin, 8 x 2 mg• PBS Pack (makes 500mL), 1 pack• Zeba Spin Desalting Columns (7K MWCO), 5 mL, 10 columns• HABA Solution, 1 mL• Avidin, 10mgStore avidin and microtubes of biotin reagent at -20°C. Store PBS at room temperature. Store remaining kit components at 4°C.
Product Line: EZ-Link™
Spacer: Pegylated
Quantity: 8 Reaction Kit
Labeling Scale: 1 to 10 mg
Label or Dye: Biotin
Labeling Target: Antibodies, Proteins
Product Type:
NHS-PEG4 Biotinylation Kit
Content And Storage:
Sufficient For: 8 labeling reactions, each with 1 to 10 mg of antibody• EZ-Link NHS-PEG4-Biotin, 8 x 2 mg• PBS Pack (makes 500mL), 1 pack• Zeba Spin Desalting Columns (7K MWCO), 5 mL, 10 columns• HABA Solution, 1 mL• Avidin, 10mgStore avidin and microtubes of biotin reagent at -20°C. Store PBS at room temperature. Store remaining kit components at 4°C.
Product Line:
EZ-Link™
Spacer:
Pegylated
Quantity:
8 Reaction Kit
Labeling Scale:
1 to 10 mg
Label or Dye:
Biotin
Labeling Target:
Antibodies, Proteins
Product Type: __
Content And Storage: Sufficient For: At least 25 pull-down reactions, each using 25 μL of resin• Glutathione Agarose, 750 μL• Pull-Down Lysis Buffer, 250 mL• Glutathione, 1 g• TBS Pack (makes 500 mL), 1 pack• Pierce Spin Columns and Accessories, 27 columns• Microcentrifuge Collection Tubes, 100 tubesUpon receipt store Pierce Lysis Buffer at room temperature. Store remaining kit components at 4°C.
Product Line: Pierce™
Spacer: __
Quantity: 1 Kit
Labeling Scale: __
Label or Dye: __
Labeling Target: __
Product Type:
__
Content And Storage:
Sufficient For: At least 25 pull-down reactions, each using 25 μL of resin• Glutathione Agarose, 750 μL• Pull-Down Lysis Buffer, 250 mL• Glutathione, 1 g• TBS Pack (makes 500 mL), 1 pack• Pierce Spin Columns and Accessories, 27 columns• Microcentrifuge Collection Tubes, 100 tubesUpon receipt store Pierce Lysis Buffer at room temperature. Store remaining kit components at 4°C.
Product Line:
Pierce™
Spacer:
__
Quantity:
1 Kit
Labeling Scale:
__
Label or Dye:
__
Labeling Target:
__
Product Type: Crosslinker
Content And Storage: Upon receipt store desiccated at 4°C.
Product Line: Pierce™
Spacer: Medium (10 to 30 Å)
Quantity: 1 g
Labeling Scale: __
Label or Dye: __
Labeling Target: __
Product Type:
Crosslinker
Content And Storage:
Upon receipt store desiccated at 4°C.
Product Line:
Pierce™
Spacer:
Medium (10 to 30 Å)
Quantity:
1 g
Labeling Scale:
__
Label or Dye:
__
Labeling Target:
__