Thermo Scientific™ SM(PEG)12 (PEGylated, long-chain SMCC crosslinker)

Description

• Thermo Scientific™ Pierce SM(PEG)n is a series of amine-to-sulfhydryl crosslinkers that differ in length from 17.6 to 95.2 Ås as a result of polyethylene glycol spacer arms containing n equals 2 to 24 ethylene glycol units
• The SM(PEG) n reagents are six crosslinkers that have identical amine- and sulfhydryl-reactive groups but differ in length as a result of spacer arms composed of different numbers of ethylene glycol units
• Their structures (shown below) have the form NHS-(PEG) n -Maleimide, where n represents 2, 4, 6, 8, 12 or 24 polyethylene glycol (PEG) units
• Each SM(PEG) n reagent is produced at the specified length without contamination from analogs of other lengths.The availability of this set of reagents makes it possible to optimize crosslinking experiments to obtain the best conjugate efficiency and function for the intended application or analysis method
• Considered together with AMAS (Part No
• 22295) and the popular SMCC (Part No
• 22360), these reagents allow for amine-to-sulfhydryl crosslinking across spaces ranging from 4.4 to 95.2Å
• In addition, the hydrophilic PEG content of SM(PEG) n reagents adds to the aqueous solubility of these crosslinkers and their conjugates
• PEG is also less immunogenic than other types of crosslinker spacers, making these reagents ideal for immunogen preparation
• Highlights: Homologous series of increasing spacer arm length, ideal for optimizing conjugation of protein interactions Water-soluble polyethylene glycol (PEG) spacer arms help to maintain or increase solubility of crosslinked proteins Reagents readily dissolve in DMSO for subsequent dilution in aqueous reaction buffers Reactive groups: NHS ester and maleimide Reactive toward: amino and sulfhydryl groups NHS-PEG 2 -Maleimide Two ethylene oxide subunits Alternate names: Mal-dPEG 2 -NHS, NHS-PEG 2 -Maleimide Molecular formula C 18 H 23 N 3 O 9 Spacer arm length 17.6Å Mass added to target per crosslink 310.12 MW 425.39 NHS-PEG 4 -Maleimide Four ethylene oxide subunits Alternate names Mal-dPEG 4 -NHS, NHS-PEG 4 -Maleimide Molecular formula C 22 H 31 N 3 O 11 Spacer arm length 24.6Å Mass added to target per crosslink 398.17 MW 513.50 NHS-PEG 6 -Maleimide Six ethylene oxide subunits Alternate names Mal-dPEG 6 -NHS, NHS-PEG 6 -Maleimide Molecular formula C 26 H 39 N 3 O 13 Spacer arm length 32.25Å Mass added to target per crosslink 486.20 MW 601.60 NHS-PEG 8 -Maleimide Eight ethylene oxide subunits Alternate names Mal-dPEG 8 -NHS, NHS-PEG 8 -Maleimide Molecular formula C 30 H 47 N 3 O 15 Spacer arm length 39.2Å Mass added to target per crosslink 574.27 MW 689.71 NHS-PEG 12 -Maleimide 12 ethylene oxide subunits Mal-dPEG 12 -NHS, NHS-PEG 12 -Maleimide Molecular Formula C 38 H 63 N 3 O 19 Spacer arm length 53.4Å Mass added to target per crosslink 750.38 MW 865.92