PI23456
Thermo Scientific™ Pierce™ Controlled Protein-Protein Crosslinking Kit
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI23456-Each-of-1 | In Stock | ₹ 62,972.16 |
PI23456 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 62,972.16
GST (18%): ₹ 11,334.989
Total Price: ₹ 74,307.149
Chemical Reactivity
Amine-Sulfhydryl
Description
Controlled Protein-Protein Crosslinking Kit
PEGylated
No
Content And Storage
Sufficient For: One conjugation with two purified proteins (up to 5 mg each)• Sulfo-SMCC Crosslinking Agent, 2 mg• Activation/Conjugation Buffer (10X), 20 mL• PBS packs (each makes 500mL), 2 packs• 2-Mercaptoethylamine-HCl, 6 mg• Immobilized-Reductant, 0.2 mL• SATA, 2 mg• Dimethylformamide (DMF), 1 mL• Hydroxylamine-HCl, 5 mg• Dextran Desalting Column, 2 x 10 mL• Column Extender, 2 ea.• Cysteine-HCl, 20 mg• Ellman's Reagent, 2 mgStore at 4°C.
Shipping Condition
Ambient
Labeling Method
Chemical Labeling
Reactive Moiety
Maleimide, Sulfo-NHS Ester
Form
Kit
Solubility
Water
Water Soluble
Yes
Cleavable
No
Molecular Weight (g/mol)
436.37
Spacer Arm Length
11.6 Å
Cell Permeability
No
Product Line
Pierce™
Crosslinker Type
Heterobifunctional
Spacer
Short (<10 Å)
Quantity
1 Reaction
Format
Kit
Product Type
Crosslinker
Related Products
Description
- Thermo Scientific™ Pierce Controlled Protein-Protein Crosslinking Kit contains essential crosslinkers, modification reagents and buffers for conjugating antibodies, enzymes and other purified proteins needed as probes
- The kit provides Sulfo-SMCC crosslinker for amine-to-sulfhydryl conjugation and SATA modification reagent for specific sulfhydryl-addition
- The crosslinking strategy allows two purified proteins, such as an antibody and a reporter enzyme, to be conjugated in a two-step, controlled manner
- Supplementary components, buffers and columns are also included to support successful creation of a useful conjugate via any one of several specific protocols
- Highlights: Sulfo-SMCC – high-quality crosslinker conjugates primary amines of protein 1 to sulfhydryl groups of protein 2 SATA or 2-MEA – modification reagents provide two options for making sulfhydryl groups available on protein 2 (usually an antibody) Ellman's Reagent – provides a mean of monitoring and assessing efficiency of the final reaction Complete – includes critical reagents as well as buffers, desalting columns and supplementary modification reagents
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Chemical Reactivity: Amine-Sulfhydryl
Description: Controlled Protein-Protein Crosslinking Kit
PEGylated: No
Content And Storage: Sufficient For: One conjugation with two purified proteins (up to 5 mg each)• Sulfo-SMCC Crosslinking Agent, 2 mg• Activation/Conjugation Buffer (10X), 20 mL• PBS packs (each makes 500mL), 2 packs• 2-Mercaptoethylamine-HCl, 6 mg• Immobilized-Reductant, 0.2 mL• SATA, 2 mg• Dimethylformamide (DMF), 1 mL• Hydroxylamine-HCl, 5 mg• Dextran Desalting Column, 2 x 10 mL• Column Extender, 2 ea.• Cysteine-HCl, 20 mg• Ellman's Reagent, 2 mgStore at 4°C.
Shipping Condition: Ambient
Labeling Method: Chemical Labeling
Reactive Moiety: Maleimide, Sulfo-NHS Ester
Form: Kit
Solubility: Water
Water Soluble: Yes
Cleavable: No
Molecular Weight (g/mol): 436.37
Spacer Arm Length: 11.6 Å
Cell Permeability: No
Product Line: Pierce™
Crosslinker Type: Heterobifunctional
Spacer: Short (<10 Å)
Quantity: 1 Reaction
Format: Kit
Product Type: Crosslinker
Chemical Reactivity:
Amine-Sulfhydryl
Description:
Controlled Protein-Protein Crosslinking Kit
PEGylated:
No
Content And Storage:
Sufficient For: One conjugation with two purified proteins (up to 5 mg each)• Sulfo-SMCC Crosslinking Agent, 2 mg• Activation/Conjugation Buffer (10X), 20 mL• PBS packs (each makes 500mL), 2 packs• 2-Mercaptoethylamine-HCl, 6 mg• Immobilized-Reductant, 0.2 mL• SATA, 2 mg• Dimethylformamide (DMF), 1 mL• Hydroxylamine-HCl, 5 mg• Dextran Desalting Column, 2 x 10 mL• Column Extender, 2 ea.• Cysteine-HCl, 20 mg• Ellman's Reagent, 2 mgStore at 4°C.
Shipping Condition:
Ambient
Labeling Method:
Chemical Labeling
Reactive Moiety:
Maleimide, Sulfo-NHS Ester
Form:
Kit
Solubility:
Water
Water Soluble:
Yes
Cleavable:
No
Molecular Weight (g/mol):
436.37
Spacer Arm Length:
11.6 Å
Cell Permeability:
No
Product Line:
Pierce™
Crosslinker Type:
Heterobifunctional
Spacer:
Short (<10 Å)
Quantity:
1 Reaction
Format:
Kit
Product Type:
Crosslinker
Chemical Reactivity: Amine
Description: __
PEGylated: __
Content And Storage: Sufficient For: One modification experiment with 0.5 to 5 mg of purified protein• SATA, 2 mg• Hydroxylamine·HCl, 5 mg• Conjugation Buffer Stock (10X), 20 mL• PBS pack (makes 500 mL), 1 pack• Dimethylformamide (DMF), 1 mL• Dextran Desalting Column, 1 x 5 mL• Column Extender, 1• Ellman's Reagent (DTNB), 2 mg• Cysteine·HCl, 20 mgStore at 4°C.
Shipping Condition: __
Labeling Method: __
Reactive Moiety: Active Ester, Succinimidyl Ester, NHS Ester
Form: Powder
Solubility: DMF, DMSO
Water Soluble: __
Cleavable: __
Molecular Weight (g/mol): __
Spacer Arm Length: __
Cell Permeability: __
Product Line: Pierce™
Crosslinker Type: Heterobifunctional
Spacer: Short (<10 Å)
Quantity: 1 Reaction
Format: __
Product Type: Crosslinker
Chemical Reactivity:
Amine
Description:
__
PEGylated:
__
Content And Storage:
Sufficient For: One modification experiment with 0.5 to 5 mg of purified protein• SATA, 2 mg• Hydroxylamine·HCl, 5 mg• Conjugation Buffer Stock (10X), 20 mL• PBS pack (makes 500 mL), 1 pack• Dimethylformamide (DMF), 1 mL• Dextran Desalting Column, 1 x 5 mL• Column Extender, 1• Ellman's Reagent (DTNB), 2 mg• Cysteine·HCl, 20 mgStore at 4°C.
Shipping Condition:
__
Labeling Method:
__
Reactive Moiety:
Active Ester, Succinimidyl Ester, NHS Ester
Form:
Powder
Solubility:
DMF, DMSO
Water Soluble:
__
Cleavable:
__
Molecular Weight (g/mol):
__
Spacer Arm Length:
__
Cell Permeability:
__
Product Line:
Pierce™
Crosslinker Type:
Heterobifunctional
Spacer:
Short (<10 Å)
Quantity:
1 Reaction
Format:
__
Product Type:
Crosslinker
Chemical Reactivity: __
Description: __
PEGylated: __
Content And Storage: Sufficient For: 10 mini gel or blot experiments with a biotinylated-protein probe• Streptavidin-HRP Conjugate, 0.1 mg• Dilution Buffer (10X), 50 mL• PBS Packs (each makes 500 mL), 17 packs• Luminol Enhancer, 55 mL• Stable Peroxide Buffer, 55 mL• Surfact-Amps 20 Detergent Solution, 6 x 10 mL• Pre-Cut Cellophane, 10 sheetsStore kit at 4°C.
Shipping Condition: __
Labeling Method: __
Reactive Moiety: __
Form: __
Solubility: __
Water Soluble: __
Cleavable: __
Molecular Weight (g/mol): __
Spacer Arm Length: __
Cell Permeability: __
Product Line: Pierce™
Crosslinker Type: __
Spacer: __
Quantity: 220 mL
Format: __
Product Type: Western Blot Kit
Chemical Reactivity:
__
Description:
__
PEGylated:
__
Content And Storage:
Sufficient For: 10 mini gel or blot experiments with a biotinylated-protein probe• Streptavidin-HRP Conjugate, 0.1 mg• Dilution Buffer (10X), 50 mL• PBS Packs (each makes 500 mL), 17 packs• Luminol Enhancer, 55 mL• Stable Peroxide Buffer, 55 mL• Surfact-Amps 20 Detergent Solution, 6 x 10 mL• Pre-Cut Cellophane, 10 sheetsStore kit at 4°C.
Shipping Condition:
__
Labeling Method:
__
Reactive Moiety:
__
Form:
__
Solubility:
__
Water Soluble:
__
Cleavable:
__
Molecular Weight (g/mol):
__
Spacer Arm Length:
__
Cell Permeability:
__
Product Line:
Pierce™
Crosslinker Type:
__
Spacer:
__
Quantity:
220 mL
Format:
__
Product Type:
Western Blot Kit
Chemical Reactivity: __
Description: __
PEGylated: __
Content And Storage: __
Shipping Condition: __
Labeling Method: __
Reactive Moiety: __
Form: __
Solubility: __
Water Soluble: __
Cleavable: __
Molecular Weight (g/mol): __
Spacer Arm Length: __
Cell Permeability: __
Product Line: __
Crosslinker Type: __
Spacer: __
Quantity: __
Format: __
Product Type: __
Chemical Reactivity:
__
Description:
__
PEGylated:
__
Content And Storage:
__
Shipping Condition:
__
Labeling Method:
__
Reactive Moiety:
__
Form:
__
Solubility:
__
Water Soluble:
__
Cleavable:
__
Molecular Weight (g/mol):
__
Spacer Arm Length:
__
Cell Permeability:
__
Product Line:
__
Crosslinker Type:
__
Spacer:
__
Quantity:
__
Format:
__
Product Type:
__