Thermo Scientific™ SDA (NHS-Diazirine) (succinimidyl 4,4'-azipentanoate)

Description

• Thermo Scientific™ Pierce NHS-Diazirine Crosslinkers combine proven NHS-ester and diazirine-based photoreaction chemistries to conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation
• The succinimidyl-ester diazirine (SDA) reagents are a new class of crosslinkers that combine proven amine-reactive chemistry with an innovative and efficient diazirine-based photochemistry for conjugating amine-containing molecules to nearly any other functional group
• The SDA crosslinkers include six compounds differing in spacer arm lengths, ability to cleave the crosslinked proteins, and presence or absence of a charged group for membrane permeability
• Protein crosslinking is an important technique used to understand protein structure and to stabilize protein-protein interactions
• Thermo Scientific SDA Reagents extend the efficiency and range of interactions that can be explored by this approach
• Highlights: Versatile - different amine-reactive diazirine derivatives capture specific intracellular or cell-surface protein interactions Controllable - two-step chemical crosslinking is activated using common laboratory UV lamps Reversible - disulfide-containing spacers enable cleavage using reducing agents Easy - reagents are photo-stable under typical laboratory lighting conditions so there is no need to perform experiments in the dark Better than aryl azides - diazirine photo-reactive group has better photostability and is more efficiently activated by long-wave UV light