Thermo Scientific™ Pierce™ Immobilized TCEP Disulfide Reducing Gel

Description

• Thermo Scientific™ Pierce Immobilized TCEP Disulfide Reducing Gel is Tris(2-carboxyethyl)phosphine that is covalently immobilized to beaded agarose resin to enable efficient reduction of peptide and protein disulfide bonds
• Trialkylphosphines serve as excellent agents for the reduction of disulfide bonds in proteins, peptides and other disulfide bond-containing molecules and are relatively unreactive toward other functional groups
• The trialkylphosphine TCEP is stable in aqueous solutions and does not undergo the rapid oxidation that often occurs with other reducing agents such as dithiotreitol (DTT) and β-mercaptoethanol (BME)
• TCEP does not interfere with common sulfhydryl-reactive reagents (e.g., maleimide crosslinkers)
• Nevertheless, many protocols require recovery of the reduced sample separate from the reducing agent
• Immobilized TCEP Disulfide Reducing Gel eliminates the need to use laborious and inefficient gel filtration methods to separate the reduced sample from the reducing agent
• Immobilized TCEP Disulfide Reducing Gel may be adapted conveniently to a variety of scales and formats (i.e
• batch, spin column and gravity-flow column procedures)
• Highlights: Odorless: reduce proteins at your desktop; contributes to a healthier lab environment Stable: virtually no loss of activity when stored refrigerated Versatile: reduces peptides and proteins over a broad range of pH, salt, detergent and temperature conditions Flexible: resin slurry provides flexibility for use in spin tube, column or batch modes Time-saving: no soluble reductant removal saves time and conserves sample Efficient: reverses both natural disulfide bonds and disulfide bonds artificially incorporated by crosslinking; excellent alternative for uncoupling crosslinked proteins or peptides by agents such as DSP and DTSSP