Thermo Scientific™ EZ-Link™ Phosphine-PEG3-Biotin

Description

• Thermo Scientific™ Pierce Biotin-PEG3-Phosphine is a biotinylation reagent for labeling azide-containing molecules, which enables biotin-based detection and affinity purification of molecules via Staudinger ligation strategies
• The phosphine group reacts with an azide to produce an aza-ylide intermediate that is trapped to form a stable, covalent amide bond, which is also referred to as the Staudinger reaction
• The azide group, which is the reactive target of phosphine-activated biotin, can be introduced into proteins or other cellular targets via azide reagents or through in vivo labeling with azide-derivatives of naturally occurring metabolic building blocks
• Because neither phosphines nor azides are present in biological systems, they comprise chemoselective (mutually specific) ligation pairs for labeling and conjugation
• Thus, when used in combination with azide labeling strategies, Phosphine-PEG 3 -Biotin enables directed biotin labeling to facilitate detection or affinity purification of protein interactions and post-translational modifications using streptavidin probes or streptavidin agarose resins
• Highlights: Soluble easily dissolves in water-miscible solvents (e.g., DMSO) for subsequent dilution in aqueous reaction mixtures with cell lysates and other biological samples Compatible reaction chemistry occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents Chemoselective the phosphine reactive group is specific in biological samples for azide-tagged molecules, ensuring that biotinylation is specific PEG spacer polyethylene glycol spacer arm helps maintain solubility of labeled molecules and decreases steric hindrance for affinity-binding to avidin, streptavidin or NeutrAvidin Protein