Thermo Scientific™ EZ-Link™ Hydrazide-PEG4-Desthiobiotin, No-Weigh™ Format

Description

• Pegylated, carbonyl-reactive labeling reagent whose biotin-like group is elutable from streptavidin, enabling its use for cell surface glycoprotein labeling and purification
• Desthiobiotin - analog of biotin allows easy elution from streptavidin, an ideal feature for affinity purification applications Glycoprotein labeling - tag glycosylated proteins at sialic acid residues to enable high recovery in pull-down assays with streptavidin beads Cell surface labeling - tag and isolate cell surface glycoproteins; reagent does not permeate membranes of whole cells Aldehyde-reactive - reacts with aldehydes formed by periodate-oxidation of sugar groups Hydrazide-activated - perform reactions at pH 4 to 6 in buffers such as sodium acetate Pegylated - spacer arm contains a hydrophilic, 4-unit, polyethylene glycol (PEG) group Enhances solubility - pegylation imparts water solubility to the biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution