Thermo Scientific™ TMTpro 18-plex, TMTpro-134C, and TMTpro-135N Label Reagents

Description

• TMTpro label reagents are the next generation of tandem mass tags, designed to increase the level of sample multiplexing without compromising on protein identification and quantitation
• The Thermo Scientific TMTpro 18plex Label Reagent Set enables multiplexing up to 18 samples, enabling even greater throughput for protein identification and quantitative analysis by tandem mass spectrometry (MS)
• The structures of the TMTpro and TMT tags are similar in being isobaric and amine reactive, but the TMTpro tag has a longer spacer region and isobutyl proline mass reporter
• After MS/MS fragmentation, each TMTpro 18plex tag generates a unique reporter mass (e.g., TMTpro 126-135 Da) in the low-mass region of the high-resolution MS/MS spectrum that is used for relative quantitation of protein expression levels
• The TMT pro134C and TMTpro135N Label Reagents are identical in composition and structure to the other TMTpro 16plex tags, but are not isobaric
• Addition of the TMTpro 134C and 135N tags create a 17th and 18th channel for relative quantitation using high resolution Orbitrap MS instruments
• These tags are available separately (Cat
• No
• A52046, A52048), as part of the TMTpro 18plex Label Reagent Set, or as part of the complete 18plex set of reagents in a 96-well plate format
• Features of the TMTpro 18plex Label Reagent Set include: • Multiplex —concurrent MS analysis of up to 18 samples derived from cells, tissues, or biological fluids • Robust —increased multiplex capability results in fewer missing quantitative values among samples and higher confidence among replicates • Efficient —amine-reactive, NHS ester-activated reagents ensure greater than 95% labeling of all peptides regardless of protein sequence or proteolytic enzyme specificity • Optimized for use —with high resolution Thermo Scientific MS/MS Orbitrap platforms, analysis fully supported by Proteome Discoverer 2.3 • Convenient size —provided in a ready-to-use, single-use format to ensure optimal stability and performance For high resolution analysis of TMTpro-labeled peptides, the recommended LC column for the Nanospray Flex source is the Acclaim PepMap 100 C18 LC Column (Cat
• No
• 164942 or 164939)
• For the EASY-Spray source, the recommended LC column is the EASY-Spray C18 LC Column (Cat
• No
• ES902 or ES903)
• When combined with the industry-leading, high resolution Orbitrap instruments and software, TMTpro reagents provide an integrated total solution for quantitative protein expression analysis.