Invitrogen™ Zenon™ Alexa Fluor™ Plus IgG Labeling Reagents

Description

• Streamline therapeutic antibody discovery campaigns with Zenon Alexa Fluor Plus Labeling Reagents to screen for antibody binding
• These reagents provide rapid, scalable antibody labeling and give a bright fluorescence-based indication of antibody binding when analyzed by flow cytometry, fluorescence microscopy, or high-content screening
• An effective cell-based screening model is critical for the success of therapeutic antibody discovery campaigns
• This serves as an important filter in the early stages of drug development
• Identifying antibodies with optimal binding profiles accelerates candidate selection and helps minimize false positives
• The new Zenon Alexa Fluor Plus Labeling Reagents are prelabeled Fab fragments directed against the Fc portion of IgG antibodies
• They are improved in three ways: superior pan-reactive Fab fragments, optimized bioconjugation, and next-generation fluorophores
• These combine to provide superior signal to background, providing more confidence in screening results
• The Zenon Alexa Fluor Plus IgG Labeling Reagents contain sufficient material to label one to four 96-well plates, depending on the Zenon labeling reagent concentration used
• Formation of the Fab–antibody complex occurs in less than 5 minutes
• Only a small amount of starting material is needed, and the method is optimized for efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions
• The insights provided by these approaches have the potential to accelerate biotherapeutic lead generation and development
• Advantages of Zenon Alexa Fluor Plus IgG Labeling Reagents include: • Simplicity —No reagent purification is required • Accuracy —No wash steps or quenchers are needed, maximizing throughput • Speed —Labeled antibodies are typically ready to use in 5 minutes • Specificity —Dyes are designed for the specific detection of antibody binding Preserve primary antibody function and affinity Reactive dye labeling of primary antibodies can have unpredictable and undesirable outcomes
• One of these is reduced binding affinity due to label addition in the binding pocket
• The Zenon antibody labeling approach, which is targeted to the Fc tail, resolves this potential issue
• Try custom antibody conjugates If the antibody conjugate you need is not available as one of our stocked products, we will prepare a custom antibody conjugate for you
• Our custom conjugation service is efficient and confidential, and we stand by the quality of our work
• We are ISO 9001:2000 certified.