Novus Biologicals™ Europium Mini Beads Antibody Conjugation Kit

-20°C

4 or 10 Mini vials of 200nm Europium (dependent on kit), 1 vial of 1x Reaction Buffer A, 1 vial of 1x Reaction Buffer B, 1 vial of 10x Europium Quencher, 1 vial of 1x Resuspension Buffer

The optimum amount of antibody (which will influence the number of antibody molecules per particle) depends on the size of the particles (surface area) and on the application. You may need to conjugate different amounts of antibody to optimize your assay. We recommend testing with 0.1 mg/mL in the first instance, although slightly lower or higher concentrations can be explored to optimize performance in your particular application. Before testing antibody concentrations above 0.1 mg/mL we advise you to use our Antibody Concentration & Clean Up Kit for LATEX and EUROPIUM to remove interfering buffer components. This is due to the increase in contamination that will occur when conjugating using a larger volume of the stock antibody. Do not alter the reaction volume of 400 ul as this will reduce conjugation efficiency.

40 μg

10 Reactions

Europium Mini Antibody Labeling Kit