PI16116
Thermo Scientific™ Active Rho Pull-Down and Detection Kit
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI16116-Each-of-1 | In Stock | ₹ 1,10,030.16 |
PI16116 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,10,030.16
GST (18%): ₹ 19,805.429
Total Price: ₹ 1,29,835.589
Content And Storage
• GST-Rhotekin-RBD, 3 vials• Anti-Rho Antibody, 5 units, 75 μL• Stabilized Goat Anti-Rabbit IgG (H+L), Peroxidase Conjugated, 2 mL• Glutathione Agarose Resin, 3 mL• GDP (100X), 50 μL• GTPyS (100X), 50 μL• SDS Sample Buffer (2X), 1.5 mL• Lysis/Binding/Wash Buffer (1X), 100 mL• Spin Cups, 30 cups• Collection Tubes, 90 tubesSee product manual for storage conditions of individual components.
Assay
Protein Quantitation Assay
Detection Method
Western Blot, Chemiluminescence
Label Type
Unlabeled
Substrate Type
GTPase Substrate
Kit Contents
Anti-Rho Antibody, 5 units, 75uL, Stabilized Goat Anti-Rabbit IgG (H+L), Peroxidase Conjugated, 2mL, Spin Cups, 30 cups, SDS Sample Buffer (2X), 1.5mL, GTPgammaS (100X), 50uL, GST-Rhotekin-RBD, 3 vials, Glutathione Agarose Resin, 3mL, Collection Tubes, 90 tubes, Lysis/Binding/Wash Buffer (1X), 100mL, GDP (100X), 50uL
Target
Kinases (Multiple), Phosphodiesterases, ATPases, GTPases, Phosphatases
Description
Pierce Active Rho Pull-Down and Detection Kit
Conjugate
HRP
Immunoassay Kit Format
Spin Column
Substrate Properties
Protein-Based Substrate
For Use With (Application)
Pull-Down
Quantity
30 reactions
Related Products
Description
- Measure activation of small GTPases by isolating them via their specific downstream effectors with Thermo Scientific™ Pierce Active GTPase Pull-Down and Detection Kits
- The Active GTPase Pull-Down and Detection Kits measure activation of small GTPases by isolating them via their specific downstream effectors
- Kits specific for Arf1, Arf6, Cdc42, Rac1, RalA, Rap1, Ras, and Rho GTPases are offered
- The respective binding domain of the downstream effector for each small GTPase is expressed as a GST-fusion protein
- When immobilized on a resin, this fusion protein is used to pull down the active (i.e., GTP-bound) GTPase
- The pull-down affinity purification step is easily and conveniently performed using microcentrifuge spin columns
- After a simple wash step, the bound GTPase is recovered by eluting the GST-fusion protein from the glutathione resin using SDS-PAGE sample loading buffer
- The purified GTPase is then detected by Western blot using a specific antibody supplied in each kit
- Each GTPase Kit includes GST-fusion protein of the specific binding domain, GTPγS and GDP activators, glutathione agarose resin, lysis-binding-wash buffer, primary antibody, 2X SDS sample buffer, spin columns and collection tubes
- Each kit contains sufficient components for 30 pull-down assays
- The kits are optimized for Western blot detection with an HRP-conjugated secondary antibody (Part No
- 31460, rabbit; Part No
- 31430, mouse) using Thermo Scientific SuperSignal™ West Pico Chemiluminescent Substrate (Part No
- 34080)
- Highlights: High sensitivity and accuracy – optimized reagents, specific antibodies and Western blot procedure ensure accurate controls and quantitative results Convenience – no need to express and purify your own GST-PBD or RBD fusion proteins or use expensive antibody-based immunoprecipitation affinity resins Speed – simultaneous incubation of cell lysate with GST-PBD or RBD and the glutathione resin directly in a high-performance microcentrifuge spin column Ease-of-use – reagents and pull-down conditions have been optimized for immediate success, even for first-time users Efficiency – the microcentrifuge spin columns and collection tubes provide efficient separation of liquid and resin, preventing sample loss Complete – kits include reagents for affinity purification as well as controls, a compatible cell lysis buffer and specific primary antibody for final Western blot detection GST fusion proteins of active GTPase binding domains: Raf1 Ras-binding domain (Raf1-RBD) binds active Ras Pak1 p21-binding domain (Pak1-PBD) binds active Rac1 and Cdc42 Rhotekin Rho-binding domain (Rhotekin-RBD) binds active Rho RalGDS Rap1-binding domain (RalGDS-RBD) binds active Rap1 GGA3 protein-binding domain (GGA3-PBD) binds active Arf1 and Arf6 Ral binding protein 1 (RalBP1) binds active RalA Includes: GST fusion protein of specific binding domain, glutathione agarose resin, GTPγS and GDP (100X), lysis-binding-wash buffer, GTPase-specific primary antibody, SDS-PAGE sample loading buffer (2X), spin columns and collection tubes
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Content And Storage: • GST-Rhotekin-RBD, 3 vials• Anti-Rho Antibody, 5 units, 75 μL• Stabilized Goat Anti-Rabbit IgG (H+L), Peroxidase Conjugated, 2 mL• Glutathione Agarose Resin, 3 mL• GDP (100X), 50 μL• GTPyS (100X), 50 μL• SDS Sample Buffer (2X), 1.5 mL• Lysis/Binding/Wash Buffer (1X), 100 mL• Spin Cups, 30 cups• Collection Tubes, 90 tubesSee product manual for storage conditions of individual components.
Assay: Protein Quantitation Assay
Detection Method: Western Blot, Chemiluminescence
Label Type: Unlabeled
Substrate Type: GTPase Substrate
Kit Contents: Anti-Rho Antibody, 5 units, 75uL, Stabilized Goat Anti-Rabbit IgG (H+L), Peroxidase Conjugated, 2mL, Spin Cups, 30 cups, SDS Sample Buffer (2X), 1.5mL, GTPgammaS (100X), 50uL, GST-Rhotekin-RBD, 3 vials, Glutathione Agarose Resin, 3mL, Collection Tubes, 90 tubes, Lysis/Binding/Wash Buffer (1X), 100mL, GDP (100X), 50uL
Target: Kinases (Multiple), Phosphodiesterases, ATPases, GTPases, Phosphatases
Description: Pierce Active Rho Pull-Down and Detection Kit
Conjugate: HRP
Immunoassay Kit Format: Spin Column
Substrate Properties: Protein-Based Substrate
For Use With (Application): Pull-Down
Quantity: 30 reactions
Content And Storage:
• GST-Rhotekin-RBD, 3 vials• Anti-Rho Antibody, 5 units, 75 μL• Stabilized Goat Anti-Rabbit IgG (H+L), Peroxidase Conjugated, 2 mL• Glutathione Agarose Resin, 3 mL• GDP (100X), 50 μL• GTPyS (100X), 50 μL• SDS Sample Buffer (2X), 1.5 mL• Lysis/Binding/Wash Buffer (1X), 100 mL• Spin Cups, 30 cups• Collection Tubes, 90 tubesSee product manual for storage conditions of individual components.
Assay:
Protein Quantitation Assay
Detection Method:
Western Blot, Chemiluminescence
Label Type:
Unlabeled
Substrate Type:
GTPase Substrate
Kit Contents:
Anti-Rho Antibody, 5 units, 75uL, Stabilized Goat Anti-Rabbit IgG (H+L), Peroxidase Conjugated, 2mL, Spin Cups, 30 cups, SDS Sample Buffer (2X), 1.5mL, GTPgammaS (100X), 50uL, GST-Rhotekin-RBD, 3 vials, Glutathione Agarose Resin, 3mL, Collection Tubes, 90 tubes, Lysis/Binding/Wash Buffer (1X), 100mL, GDP (100X), 50uL
Target:
Kinases (Multiple), Phosphodiesterases, ATPases, GTPases, Phosphatases
Description:
Pierce Active Rho Pull-Down and Detection Kit
Conjugate:
HRP
Immunoassay Kit Format:
Spin Column
Substrate Properties:
Protein-Based Substrate
For Use With (Application):
Pull-Down
Quantity:
30 reactions
Content And Storage: • GST-Raf1-RBD Fusion Protein, 1 vial• Anti-Ras Antibody, 5 units, 250 μL• Glutathione Agarose Resin, 3 mL• GDP (100X), 50 μL• GTPyS (100X), 50 μL• SDS Sample Buffer (2X), 1.5 mL• Lysis/Binding/Wash Buffer (1X), 100 mL• Spin Cups, 30 cups• Collection Tubes, 90 tubesSee product manual for storage conditions of individual components.
Assay: Protein Quantitation Assay
Detection Method: Western Blot, Chemiluminescence
Label Type: Unlabeled
Substrate Type: GTPase Substrate
Kit Contents: SDS Sample Buffer (2X), 1.5mL, Spin Cups, 30 cups, Lysis/Binding/Wash Buffer (1X), 100mL, GTPγS (100X), 50μL, GST-Raf1-RBD Fusion Protein, 1 vial, Glutathione Agarose Resin, 3mL, GDP (100X), 50µL, Anti-Ras Antibody, 5 units, 250µL, Collection Tubes, 90 tubes
Target: Kinases (Multiple), Phosphodiesterases, ATPases, GTPases, Phosphatases
Description: Pierce Active Ras Pull-Down and Detection Kit
Conjugate: HRP
Immunoassay Kit Format: Spin Column
Substrate Properties: Protein-Based Substrate
For Use With (Application): Pull-Down
Quantity: 30 reactions
Content And Storage:
• GST-Raf1-RBD Fusion Protein, 1 vial• Anti-Ras Antibody, 5 units, 250 μL• Glutathione Agarose Resin, 3 mL• GDP (100X), 50 μL• GTPyS (100X), 50 μL• SDS Sample Buffer (2X), 1.5 mL• Lysis/Binding/Wash Buffer (1X), 100 mL• Spin Cups, 30 cups• Collection Tubes, 90 tubesSee product manual for storage conditions of individual components.
Assay:
Protein Quantitation Assay
Detection Method:
Western Blot, Chemiluminescence
Label Type:
Unlabeled
Substrate Type:
GTPase Substrate
Kit Contents:
SDS Sample Buffer (2X), 1.5mL, Spin Cups, 30 cups, Lysis/Binding/Wash Buffer (1X), 100mL, GTPγS (100X), 50μL, GST-Raf1-RBD Fusion Protein, 1 vial, Glutathione Agarose Resin, 3mL, GDP (100X), 50µL, Anti-Ras Antibody, 5 units, 250µL, Collection Tubes, 90 tubes
Target:
Kinases (Multiple), Phosphodiesterases, ATPases, GTPases, Phosphatases
Description:
Pierce Active Ras Pull-Down and Detection Kit
Conjugate:
HRP
Immunoassay Kit Format:
Spin Column
Substrate Properties:
Protein-Based Substrate
For Use With (Application):
Pull-Down
Quantity:
30 reactions
Content And Storage: • GST-Pak1-PBD Fusion Protein, 1 vial• Anti-Rac1 Antibody, 5 units, 50 μL• Glutathione Agarose Resin, 3 mL• GDP (100X), 50 μL• GTPyS (100X), 50 μL• SDS Sample Buffer (2X), 1.5 mL• Lysis/Binding/Wash Buffer (1X), 100 mL• Spin Cups, 30 cups• Collection Tubes, 90 tubesSee product manual for storage conditions of individual components.
Assay: Protein Quantitation Assay
Detection Method: Western Blot, Chemiluminescence
Label Type: Unlabeled
Substrate Type: GTPase Substrate
Kit Contents: Spin Cups, 30 cups, SDS Sample Buffer (2X), 1.5mL, Lysis/Binding/Wash Buffer (1X), 100mL, GTPγS (100X), 50μL, GST-Pak1-PBD Fusion Protein, 1 vial, Glutathione Agarose Resin, 3mL, GDP (100X), 50µL, Collection Tubes, 90 tubes, Anti-Rac1 Antibody, 5 units, 50µL
Target: Kinases (Multiple), Phosphodiesterases, ATPases, GTPases, Phosphatases
Description: Pierce Active Rac1 Pull-Down and Detection Kit
Conjugate: HRP
Immunoassay Kit Format: Spin Column
Substrate Properties: Protein-Based Substrate
For Use With (Application): Pull-Down
Quantity: 30 reactions
Content And Storage:
• GST-Pak1-PBD Fusion Protein, 1 vial• Anti-Rac1 Antibody, 5 units, 50 μL• Glutathione Agarose Resin, 3 mL• GDP (100X), 50 μL• GTPyS (100X), 50 μL• SDS Sample Buffer (2X), 1.5 mL• Lysis/Binding/Wash Buffer (1X), 100 mL• Spin Cups, 30 cups• Collection Tubes, 90 tubesSee product manual for storage conditions of individual components.
Assay:
Protein Quantitation Assay
Detection Method:
Western Blot, Chemiluminescence
Label Type:
Unlabeled
Substrate Type:
GTPase Substrate
Kit Contents:
Spin Cups, 30 cups, SDS Sample Buffer (2X), 1.5mL, Lysis/Binding/Wash Buffer (1X), 100mL, GTPγS (100X), 50μL, GST-Pak1-PBD Fusion Protein, 1 vial, Glutathione Agarose Resin, 3mL, GDP (100X), 50µL, Collection Tubes, 90 tubes, Anti-Rac1 Antibody, 5 units, 50µL
Target:
Kinases (Multiple), Phosphodiesterases, ATPases, GTPases, Phosphatases
Description:
Pierce Active Rac1 Pull-Down and Detection Kit
Conjugate:
HRP
Immunoassay Kit Format:
Spin Column
Substrate Properties:
Protein-Based Substrate
For Use With (Application):
Pull-Down
Quantity:
30 reactions
Content And Storage: • GST-Pak1-RBD Fusion Protein, 1 vial• Anti-Cdc42 Antibody, 5 units, 300 μL• Glutathione Agarose Resin, 3 mL• GDP (100X), 50 μL• GTPyS (100X), 50 μL• SDS Sample Buffer (2X), 1.5 mL• Lysis/Binding/Wash Buffer (1X), 100 mL• Spin Cups, 30 cups• Collection Tubes, 90 tubesSee product manual for storage conditions of individual components.
Assay: Protein Quantitation Assay
Detection Method: Western Blot, Chemiluminescence
Label Type: Unlabeled
Substrate Type: GTPase Substrate
Kit Contents: Anti-Cdc42 Antibody, 5 units, 300µL, Collection Tubes, 90 tubes, GDP (100X), 50µL, Glutathione Agarose Resin, 3mL, GST-Pak1-RBD Fusion Protein, 1 vial, GTPγS (100X), 50μL, Lysis/Binding/Wash Buffer (1X), 100mL, SDS Sample Buffer (2X), 1.5mL, Spin Cups, 30 cups
Target: Kinases (Multiple), Phosphodiesterases, ATPases, GTPases, Phosphatases
Description: Pierce Active Cdc42 Pull-Down and Detection Kit
Conjugate: HRP
Immunoassay Kit Format: Spin Column
Substrate Properties: Protein-Based Substrate
For Use With (Application): Pull-Down
Quantity: 30 reactions
Content And Storage:
• GST-Pak1-RBD Fusion Protein, 1 vial• Anti-Cdc42 Antibody, 5 units, 300 μL• Glutathione Agarose Resin, 3 mL• GDP (100X), 50 μL• GTPyS (100X), 50 μL• SDS Sample Buffer (2X), 1.5 mL• Lysis/Binding/Wash Buffer (1X), 100 mL• Spin Cups, 30 cups• Collection Tubes, 90 tubesSee product manual for storage conditions of individual components.
Assay:
Protein Quantitation Assay
Detection Method:
Western Blot, Chemiluminescence
Label Type:
Unlabeled
Substrate Type:
GTPase Substrate
Kit Contents:
Anti-Cdc42 Antibody, 5 units, 300µL, Collection Tubes, 90 tubes, GDP (100X), 50µL, Glutathione Agarose Resin, 3mL, GST-Pak1-RBD Fusion Protein, 1 vial, GTPγS (100X), 50μL, Lysis/Binding/Wash Buffer (1X), 100mL, SDS Sample Buffer (2X), 1.5mL, Spin Cups, 30 cups
Target:
Kinases (Multiple), Phosphodiesterases, ATPases, GTPases, Phosphatases
Description:
Pierce Active Cdc42 Pull-Down and Detection Kit
Conjugate:
HRP
Immunoassay Kit Format:
Spin Column
Substrate Properties:
Protein-Based Substrate
For Use With (Application):
Pull-Down
Quantity:
30 reactions