Novus Biologicals™ GTPase Activity Kit (Colorimetric)

Description

• Colorimetric assays for GTPases are invariably based on the formation of colored complexes between an inorganic phosphate and a dye molecule under acidic conditions
• Such assays are beset with problems of reagent precipitation and high backgrounds caused by impure substrates (i.e
• contaminated with inorganic phosphate) and/or by non-enzymatic (acid) hydrolysis of the substrate
• At the heart of our assay kits is PiColorLock Gold, a superior phosphate detection reagent which ensures high stability of the colored dye-phosphate complexes
• Together with specially purified substrates and proprietary stabilizers our kits offer the lowest possible assay backgrounds and outstanding assay performance
• Uniquely, Innova Biosciences GTPase assay kits contain highly purified GTP, which have had any free inorganic phosphate removed - this means that assays have the lowest possible background signal
• PiColorLock Phosphate Detection Systems can also be purchased for developing simple assays for any phosphate-generating enzyme
• This non radioactive colorimetric assay kit uses a 96 well format, and all the necessary reagents are supplied for measuring enzyme activity and are ideal for high throughput drug screening
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