7070050

Novus Biologicals™ Lightning-Link (R) APC-Cy5.5 Antibody Labeling Kit

Antibody labeling kit

Manufacturer: Fischer Scientific

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Content And Storage

Store at -20°C. Avoid freeze-thaw cycles.

Conjugate

APC/Cy5.5

Kit Contents

Glass vial(s) of Lightning-Link mix (1 or 3 vials, depending on pack size), 1 vial of LL Modifier reagent, 1 vial of LL Quencher FD reagent

Product Type

APC-Cy5.5 Antibody Labeling Kit

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Description

  • The antibody is covalently bonded to the label in a directional and controlled process at near-neutral pH
  • APC/Cy5.5 is a tandem conjugate
  • In the Lightning-Link APC/Cy5.5 the tandem is excited at 633nm, wavelength at which the APC has its maximum absorption
  • The excited APC functions then as an energy donor, where the energy is transferred via FRET (Fluorescence Resonance Energy Transfer) to the Cy5.5
  • The Cy5.5 emits then the energy received from the APC in the form of long wavelength light at 694nm
  • The amount of antibody used for labeling should be the same as the pack size of LL-APC-Cy5.5
  • For any new antibody, optimization of the ratio of antibody to dye is often worthwhile
  • If your antibody needs to be concentrated, purified, or cleaned up, consider purchasing an antibody clean up kit.

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Content And Storage:
Store at -20°C. Avoid freeze-thaw cycles.

Conjugate:
APC/Cy5.5

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Glass vial(s) of Lightning-Link mix (1 or 3 vials, depending on pack size), 1 vial of LL Modifier reagent, 1 vial of LL Quencher FD reagent

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