7070046
Novus Biologicals™ Lightning-Link (R) PE-Cy5 Antibody Labeling Kit
Applications:Flow Cytometry
Manufacturer: Fischer Scientific
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Content And Storage
Store at -20°C. Avoid freeze-thaw cycles.
Conjugate
PE/Cy5
Kit Contents
Glass vial(s) of Lightning-Link mix (1 or 3 vials, depending on pack size), 1 vial of LL Modifier reagent, 1 vial of LL Quencher FD reagent
Product Type
PE-Cy5 Antibody Labeling Kit
Related Products
Description
- PE/Cy5 is a tandem conjugate
- The PE is excited at 488nm and functions as an energy donor for the Cy5
- The energy is transferred via FRET (Fluorescence Resonance Energy Transfer) to the Cy5, which emits then the energy received in the form of long wavelength light at 670nm
- The antibody is covalently bonded to the label in a directional and controlled process at near-neutral pH
- In view of the large size of the PE-Cy5 label, the amount of antibody used in a labeling reaction with the tandem must always be less than the pack size of LL PE-Cy5, in order that the PE-Cy5 is in a slight molar excess
- The best ratio for any new antibody reagent must be determined by experimentation but 50-60ug of IgG antibody for every 100ug of LL PE-Cy5 usually gives optimal results
- 50ug of antibody corresponds to an Ab:PE-Cy5 molar ratio of 1:1
- The amount of antibody used for labeling should be the same as the pack size of LL-PE-Cy5
- For any new antibody, optimization of the ratio of antibody to dye is often worthwhile
- If your antibody needs to be concentrated, purified, or cleaned up, consider purchasing an antibody clean up kit.
Compare Similar Items
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Content And Storage: Store at -20°C. Avoid freeze-thaw cycles.
Conjugate: PE/Cy5
Kit Contents: Glass vial(s) of Lightning-Link mix (1 or 3 vials, depending on pack size), 1 vial of LL Modifier reagent, 1 vial of LL Quencher FD reagent
Product Type: PE-Cy5 Antibody Labeling Kit
Content And Storage:
Store at -20°C. Avoid freeze-thaw cycles.
Conjugate:
PE/Cy5
Kit Contents:
Glass vial(s) of Lightning-Link mix (1 or 3 vials, depending on pack size), 1 vial of LL Modifier reagent, 1 vial of LL Quencher FD reagent
Product Type:
PE-Cy5 Antibody Labeling Kit
Content And Storage: Store at -20°C. Avoid freeze-thaw cycles.
Conjugate: PE/Cy7
Kit Contents: __
Product Type: PE-Cy7 Antibody Labeling Kit
Content And Storage:
Store at -20°C. Avoid freeze-thaw cycles.
Conjugate:
PE/Cy7
Kit Contents:
__
Product Type:
PE-Cy7 Antibody Labeling Kit
Content And Storage: Store at -20°C. Avoid freeze-thaw cycles.
Conjugate: PerCP/Cy5.5
Kit Contents: Glass vial(s) of Lightning-Link mix (1 or 3 vials, depending on pack size), 1 vial of LL Modifier reagent, 1 vial of LL Quencher FD reagent
Product Type: PerCP-Cy5.5 Antibody Labeling Kit
Content And Storage:
Store at -20°C. Avoid freeze-thaw cycles.
Conjugate:
PerCP/Cy5.5
Kit Contents:
Glass vial(s) of Lightning-Link mix (1 or 3 vials, depending on pack size), 1 vial of LL Modifier reagent, 1 vial of LL Quencher FD reagent
Product Type:
PerCP-Cy5.5 Antibody Labeling Kit
Content And Storage: Store at -20°C. Avoid freeze-thaw cycles.
Conjugate: APC/Cy5.5
Kit Contents: Glass vial(s) of Lightning-Link mix (1 or 3 vials, depending on pack size), 1 vial of LL Modifier reagent, 1 vial of LL Quencher FD reagent
Product Type: APC-Cy5.5 Antibody Labeling Kit
Content And Storage:
Store at -20°C. Avoid freeze-thaw cycles.
Conjugate:
APC/Cy5.5
Kit Contents:
Glass vial(s) of Lightning-Link mix (1 or 3 vials, depending on pack size), 1 vial of LL Modifier reagent, 1 vial of LL Quencher FD reagent
Product Type:
APC-Cy5.5 Antibody Labeling Kit