76-000-15
Novus Biologicals™ Lightning-Link (R) PE-Cy5 Antibody Labeling Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 76-000-15-Each-of-1 | In Stock | ₹ 1,06,436.64 |
76-000-15 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,06,436.64
GST (18%): ₹ 19,158.595
Total Price: ₹ 1,25,595.235
Content And Storage
Store at -20°C. Avoid freeze-thaw cycles.
Conjugate
PE/Cy5
Product Type
PE-Cy5 Antibody Labeling Kit
Quantity
600 μg
Kit Contents
Glass vial(s) of Lightning-Link mix (1 or 3 vials, depending on pack size), 1 vial of LL Modifier reagent, 1 vial of LL Quencher FD reagent
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Description
- PE/Cy5 is a tandem conjugate
- The PE is excited at 488nm and functions as an energy donor for the Cy5
- The energy is transferred via FRET (Fluorescence Resonance Energy Transfer) to the Cy5, which emits then the energy received in the form of long wavelength light at 670nm
- The antibody is covalently bonded to the label in a directional and controlled process at near-neutral pH
- In view of the large size of the PE-Cy5 label, the amount of antibody used in a labeling reaction with the tandem must always be less than the pack size of LL PE-Cy5, in order that the PE-Cy5 is in a slight molar excess
- The best ratio for any new antibody reagent must be determined by experimentation but 50-60ug of IgG antibody for every 100ug of LL PE-Cy5 usually gives optimal results
- 50ug of antibody corresponds to an Ab:PE-Cy5 molar ratio of 1:1
- The amount of antibody used for labeling should be the same as the pack size of LL-PE-Cy5
- For any new antibody, optimization of the ratio of antibody to dye is often worthwhile
- If your antibody needs to be concentrated, purified, or cleaned up, consider purchasing an antibody clean up kit.
Compare Similar Items
Show Difference
Content And Storage: Store at -20°C. Avoid freeze-thaw cycles.
Conjugate: PE/Cy5
Product Type: PE-Cy5 Antibody Labeling Kit
Quantity: 600 μg
Kit Contents: Glass vial(s) of Lightning-Link mix (1 or 3 vials, depending on pack size), 1 vial of LL Modifier reagent, 1 vial of LL Quencher FD reagent
Content And Storage:
Store at -20°C. Avoid freeze-thaw cycles.
Conjugate:
PE/Cy5
Product Type:
PE-Cy5 Antibody Labeling Kit
Quantity:
600 μg
Kit Contents:
Glass vial(s) of Lightning-Link mix (1 or 3 vials, depending on pack size), 1 vial of LL Modifier reagent, 1 vial of LL Quencher FD reagent
Content And Storage: Store at -20°C. Avoid freeze-thaw cycles.
Conjugate: PE/Cy5
Product Type: PE-Cy5 Antibody Labeling Kit
Quantity: 30 μg
Kit Contents: Glass vial(s) of Lightning-Link mix (1 or 3 vials, depending on pack size), 1 vial of LL Modifier reagent, 1 vial of LL Quencher FD reagent
Content And Storage:
Store at -20°C. Avoid freeze-thaw cycles.
Conjugate:
PE/Cy5
Product Type:
PE-Cy5 Antibody Labeling Kit
Quantity:
30 μg
Kit Contents:
Glass vial(s) of Lightning-Link mix (1 or 3 vials, depending on pack size), 1 vial of LL Modifier reagent, 1 vial of LL Quencher FD reagent
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