PI16159
Thermo Scientific™ Pierce™ Gaussia Luciferase Flash Assay Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI16159-Each-of-1 | In Stock | ₹ 71,100.36 |
PI16159 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 71,100.36
GST (18%): ₹ 12,798.065
Total Price: ₹ 83,898.425
Type
Assay Kit
Sufficient For
1000 Luciferase Assays
Content And Storage
Sufficient For: 1000 luciferase assays in microplate wells• Gaussia Flash Assay Buffer, 50 mL (store at 4°C)• 100X Coelenterazine, 0.5 mL (store at -80°C)• Luciferase Cell Lysis Buffer (2X), 60 mL (store at room temperature)Kit may be stored at -80°C, or individually at indicated temperatures.
Compatible Cells
Mammalian Cells
For Use With (Equipment)
Luminometer (Microplate)
Label Type
Enzyme Labeled
Technique
Enhanced Chemiluminescence
Product Line
Pierce™
Target
Luciferase, Gaussia Luciferase
Quantity
1000 Reaction Kit
Assay
Reporter Enzyme, Luciferase Reporter Assay
Detection Method
Bioluminescence
Format
384-well plate, 96-well plate
Substrate Properties
Chemical Substrate
Substrate Type
Luciferase Substrate
Substrate
Coelenterazine
Related Products
Description
- The Thermo Scientific™ Pierce Gaussia Luciferase Flash Assay Kit provides researchers with the most sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate
- This Flash Assay Kit contains reagents for measuring the activity of Gaussia luciferase in mammalian cell culture media and lysates
- When used with Thermo Scientific Gaussia Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of promoter or pathway activity
- Gaussia luciferase is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity
- The signal produced by Gaussia is considerably greater than signal from either firefly or Renilla luciferases assayed under similar conditions
- Highlights: Sensitive – 1000 times greater sensitivity allows utilization of smaller numbers of cells Cost effective – highest sensitivity assays result in decreased reagent consumption Secreted luciferase – allows real-time assays and kinetic studies without destroying cells Time-saving – assays using secreted luciferases require minimal sample handling Automation-friendly – amenable to high throughput screens Convenient – contains a universal cell lysis buffer and optimized flash assay reagent Safe – allows one to perform non-radioactive assays Includes: Cell lysis buffer, reaction buffer and substrate Requires: Gaussia luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers Recommended for: Promoter studies for analyzing cis - regulatory elements and trans -acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Secretory pathway / protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies
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Type: Assay Kit
Sufficient For: 1000 Luciferase Assays
Content And Storage: Sufficient For: 1000 luciferase assays in microplate wells• Gaussia Flash Assay Buffer, 50 mL (store at 4°C)• 100X Coelenterazine, 0.5 mL (store at -80°C)• Luciferase Cell Lysis Buffer (2X), 60 mL (store at room temperature)Kit may be stored at -80°C, or individually at indicated temperatures.
Compatible Cells: Mammalian Cells
For Use With (Equipment): Luminometer (Microplate)
Label Type: Enzyme Labeled
Technique: Enhanced Chemiluminescence
Product Line: Pierce™
Target: Luciferase, Gaussia Luciferase
Quantity: 1000 Reaction Kit
Assay: Reporter Enzyme, Luciferase Reporter Assay
Detection Method: Bioluminescence
Format: 384-well plate, 96-well plate
Substrate Properties: Chemical Substrate
Substrate Type: Luciferase Substrate
Substrate: Coelenterazine
Type:
Assay Kit
Sufficient For:
1000 Luciferase Assays
Content And Storage:
Sufficient For: 1000 luciferase assays in microplate wells• Gaussia Flash Assay Buffer, 50 mL (store at 4°C)• 100X Coelenterazine, 0.5 mL (store at -80°C)• Luciferase Cell Lysis Buffer (2X), 60 mL (store at room temperature)Kit may be stored at -80°C, or individually at indicated temperatures.
Compatible Cells:
Mammalian Cells
For Use With (Equipment):
Luminometer (Microplate)
Label Type:
Enzyme Labeled
Technique:
Enhanced Chemiluminescence
Product Line:
Pierce™
Target:
Luciferase, Gaussia Luciferase
Quantity:
1000 Reaction Kit
Assay:
Reporter Enzyme, Luciferase Reporter Assay
Detection Method:
Bioluminescence
Format:
384-well plate, 96-well plate
Substrate Properties:
Chemical Substrate
Substrate Type:
Luciferase Substrate
Substrate:
Coelenterazine
Type: Assay Kit
Sufficient For: 100 Luciferase Assays
Content And Storage: Sufficient For: 100 luciferase assays in microplate wells• Gaussia Glow Assay Buffer, 5 mL (store at 4°C)• 100X Coelenterazine, 50 μL (store at -80°C)• Luciferase Cell Lysis Buffer (2X), 6 mL (store at room temperature)Kit may be stored at -80°C, or individually at indicated temperatures.
Compatible Cells: Mammalian Cells
For Use With (Equipment): Luminometer (Microplate)
Label Type: Enzyme Labeled
Technique: Enhanced Chemiluminescence
Product Line: Pierce™
Target: Luciferase, Gaussia Luciferase
Quantity: 100 Reactions
Assay: Reporter Enzyme, Luciferase Reporter Assay
Detection Method: Bioluminescence
Format: 384-well plate, 96-well plate
Substrate Properties: Chemical Substrate
Substrate Type: Luciferase Substrate
Substrate: Coelenterazine
Type:
Assay Kit
Sufficient For:
100 Luciferase Assays
Content And Storage:
Sufficient For: 100 luciferase assays in microplate wells• Gaussia Glow Assay Buffer, 5 mL (store at 4°C)• 100X Coelenterazine, 50 μL (store at -80°C)• Luciferase Cell Lysis Buffer (2X), 6 mL (store at room temperature)Kit may be stored at -80°C, or individually at indicated temperatures.
Compatible Cells:
Mammalian Cells
For Use With (Equipment):
Luminometer (Microplate)
Label Type:
Enzyme Labeled
Technique:
Enhanced Chemiluminescence
Product Line:
Pierce™
Target:
Luciferase, Gaussia Luciferase
Quantity:
100 Reactions
Assay:
Reporter Enzyme, Luciferase Reporter Assay
Detection Method:
Bioluminescence
Format:
384-well plate, 96-well plate
Substrate Properties:
Chemical Substrate
Substrate Type:
Luciferase Substrate
Substrate:
Coelenterazine
Type: Assay Kit
Sufficient For: 1000 Luciferase Assays
Content And Storage: Sufficient For: 1000 luciferase assays in microplate wells• Gaussia Glow Assay Buffer, 50 mL (store at 4°C)• 100X Coelenterazine, 0.5 mL (store at -80°C)• Luciferase Cell Lysis Buffer (2X), 60 mL (store at room temperature)Kit may be stored at -80°C, or individually at indicated temperatures.
Compatible Cells: Mammalian Cells
For Use With (Equipment): Luminometer (Microplate)
Label Type: Enzyme Labeled
Technique: Enhanced Chemiluminescence
Product Line: Pierce™
Target: Luciferase, Gaussia Luciferase
Quantity: 1000 Reactions
Assay: Reporter Enzyme, Luciferase Reporter Assay
Detection Method: Bioluminescence
Format: 384-well plate, 96-well plate
Substrate Properties: Chemical Substrate
Substrate Type: Luciferase Substrate
Substrate: Coelenterazine
Type:
Assay Kit
Sufficient For:
1000 Luciferase Assays
Content And Storage:
Sufficient For: 1000 luciferase assays in microplate wells• Gaussia Glow Assay Buffer, 50 mL (store at 4°C)• 100X Coelenterazine, 0.5 mL (store at -80°C)• Luciferase Cell Lysis Buffer (2X), 60 mL (store at room temperature)Kit may be stored at -80°C, or individually at indicated temperatures.
Compatible Cells:
Mammalian Cells
For Use With (Equipment):
Luminometer (Microplate)
Label Type:
Enzyme Labeled
Technique:
Enhanced Chemiluminescence
Product Line:
Pierce™
Target:
Luciferase, Gaussia Luciferase
Quantity:
1000 Reactions
Assay:
Reporter Enzyme, Luciferase Reporter Assay
Detection Method:
Bioluminescence
Format:
384-well plate, 96-well plate
Substrate Properties:
Chemical Substrate
Substrate Type:
Luciferase Substrate
Substrate:
Coelenterazine
Type: Assay Kit
Sufficient For: __
Content And Storage: • Renilla Flash Assay Buffer, 5 mL, store at 4°C• Coelenterazine (100X), 50 μL, store at -80°C• 2X Cell Lysis Buffer, 6 mL, store at room temperature
Compatible Cells: Mammalian Cells
For Use With (Equipment): Luminometer (Microplate)
Label Type: Enzyme Labeled
Technique: Enhanced Chemiluminescence
Product Line: Pierce™
Target: Luciferase, Green Renilla Luciferase
Quantity: 100 Reaction Kit
Assay: Reporter Enzyme, Luciferase Reporter Assay
Detection Method: Bioluminescence
Format: 384-well plate, 96-well plate
Substrate Properties: Chemical Substrate
Substrate Type: Luciferase Substrate
Substrate: Coelenterazine
Type:
Assay Kit
Sufficient For:
__
Content And Storage:
• Renilla Flash Assay Buffer, 5 mL, store at 4°C• Coelenterazine (100X), 50 μL, store at -80°C• 2X Cell Lysis Buffer, 6 mL, store at room temperature
Compatible Cells:
Mammalian Cells
For Use With (Equipment):
Luminometer (Microplate)
Label Type:
Enzyme Labeled
Technique:
Enhanced Chemiluminescence
Product Line:
Pierce™
Target:
Luciferase, Green Renilla Luciferase
Quantity:
100 Reaction Kit
Assay:
Reporter Enzyme, Luciferase Reporter Assay
Detection Method:
Bioluminescence
Format:
384-well plate, 96-well plate
Substrate Properties:
Chemical Substrate
Substrate Type:
Luciferase Substrate
Substrate:
Coelenterazine