PI16185
Thermo Scientific™ Pierce™ Renilla-Firefly Luciferase Dual Assay Kit
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI16185-Each-of-1 | In Stock | ₹ 16,846.76 |
PI16185 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 16,846.76
GST (18%): ₹ 3,032.417
Total Price: ₹ 19,879.177
Type
Assay Kit
Sufficient For
100 Dual Luciferase Assays
Content And Storage
Sufficient For: 100 dual-luciferase assays in microplate wells• Renilla-Firefly Dual Assay Buffer, 5 mL (store at 4°C)• 100X Coelenterazine, 50 μL (store at -80°C)• D-Luciferin, 3 mg (store at 4°C)• Luciferase Cell Lysis Buffer (2X), 6 mL (store at room temperature)Upon receipt, store kit at -80°C, or store individual components as indicated above.
Compatible Cells
Mammalian Cells
For Use With (Equipment)
Luminometer (Microplate)
Label Type
Enzyme Labeled
Technique
Enhanced Chemiluminescence
Product Line
Pierce™
Target
Luciferase, Firefly Luciferase, Green Renilla Luciferase
Quantity
100 Reaction Kit
Assay
Reporter Enzyme, Luciferase Reporter Assay
Detection Method
Bioluminescence
Format
384-well plate, 96-well plate
Substrate Properties
Chemical Substrate
Substrate Type
Luciferase Substrate
Substrate
Coelenterazine, D-Luciferin
Related Products
Description
- The Thermo Scientific™ Pierce Renilla-Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular Renilla and Red Firefly luciferase activity in mammalian whole cell lysates
- The Pierce Renilla-Firefly Luciferase Dual Assay Kit is a highly sensitive assay that allows for the simultaneous detection of Green Renilla and Red Firefly luciferase activity
- Green Renilla luciferase acts as an experimental reporter with constitutively active red firefly as a normalization control
- This reporter-and-control combination enables simultaneous monitoring of experimental reporter and control luciferase activities in a single-read assay without the need for two-step addition of substrate reagents or quenching
- The assay working solution contains substrates for both luciferases, and the reactions occur simultaneously with flash-type kinetics
- The resulting luminescent signals are spectrally resolvable using filters
- In a single sample, researchers can assay transcriptional activity of regulatory elements, signal transduction pathways, and effects of activators or inhibitors
- Highlights: Simultaneous – concurrent, filter-based, wavelength-separated detection of two luciferase activities Sensitive – measure Green Renilla and red firefly luciferase activities in the same sample Fast – no quenching step required, unlike in traditional sequential dual assays Multiplex – capable of quantitating two cellular activities in the same sample(s) Includes: Cell lysis buffer, reaction buffer, and substrates coelenterazine and D-luciferin Requires: Green Renilla and Red Firefly luciferase reporters; Filter sets at 525nm±20nm for Green Renilla Luc and 615 to 675nm for Red Firefly Luc
- Luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers; Injector required for assessing more than 24 wells at a time
- Recommended for: Study two regulatory elements at the same time; Monitor two signaling pathways simultaneously; Enable studying more than one target per screen (e.g
- off-target effects)
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Type: Assay Kit
Sufficient For: 100 Dual Luciferase Assays
Content And Storage: Sufficient For: 100 dual-luciferase assays in microplate wells• Renilla-Firefly Dual Assay Buffer, 5 mL (store at 4°C)• 100X Coelenterazine, 50 μL (store at -80°C)• D-Luciferin, 3 mg (store at 4°C)• Luciferase Cell Lysis Buffer (2X), 6 mL (store at room temperature)Upon receipt, store kit at -80°C, or store individual components as indicated above.
Compatible Cells: Mammalian Cells
For Use With (Equipment): Luminometer (Microplate)
Label Type: Enzyme Labeled
Technique: Enhanced Chemiluminescence
Product Line: Pierce™
Target: Luciferase, Firefly Luciferase, Green Renilla Luciferase
Quantity: 100 Reaction Kit
Assay: Reporter Enzyme, Luciferase Reporter Assay
Detection Method: Bioluminescence
Format: 384-well plate, 96-well plate
Substrate Properties: Chemical Substrate
Substrate Type: Luciferase Substrate
Substrate: Coelenterazine, D-Luciferin
Type:
Assay Kit
Sufficient For:
100 Dual Luciferase Assays
Content And Storage:
Sufficient For: 100 dual-luciferase assays in microplate wells• Renilla-Firefly Dual Assay Buffer, 5 mL (store at 4°C)• 100X Coelenterazine, 50 μL (store at -80°C)• D-Luciferin, 3 mg (store at 4°C)• Luciferase Cell Lysis Buffer (2X), 6 mL (store at room temperature)Upon receipt, store kit at -80°C, or store individual components as indicated above.
Compatible Cells:
Mammalian Cells
For Use With (Equipment):
Luminometer (Microplate)
Label Type:
Enzyme Labeled
Technique:
Enhanced Chemiluminescence
Product Line:
Pierce™
Target:
Luciferase, Firefly Luciferase, Green Renilla Luciferase
Quantity:
100 Reaction Kit
Assay:
Reporter Enzyme, Luciferase Reporter Assay
Detection Method:
Bioluminescence
Format:
384-well plate, 96-well plate
Substrate Properties:
Chemical Substrate
Substrate Type:
Luciferase Substrate
Substrate:
Coelenterazine, D-Luciferin
Type: Assay Kit
Sufficient For: 1000 Dual Luciferase Assays
Content And Storage: Sufficient For: 1000 dual-luciferase assays in microplate wells• Renilla-Firefly Dual Assay Buffer, 50 mL (store at 4°C)• 100X Coelenterazine, 0.5 mL (store at -80°C)• D-Luciferin, 30 mg (store at 4°C)• Luciferase Cell Lysis Buffer (2X), 60 mL (store at room temperature)Upon receipt, store kit at -80°C, or store individual components as indicated above.
Compatible Cells: Mammalian Cells
For Use With (Equipment): Luminometer (Microplate)
Label Type: Enzyme Labeled
Technique: Enhanced Chemiluminescence
Product Line: Pierce™
Target: Luciferase, Firefly Luciferase, Green Renilla Luciferase
Quantity: 1000 Reaction Kit
Assay: Reporter Enzyme, Luciferase Reporter Assay
Detection Method: Bioluminescence
Format: 384-well plate, 96-well plate
Substrate Properties: Chemical Substrate
Substrate Type: Luciferase Substrate
Substrate: Coelenterazine, D-Luciferin
Type:
Assay Kit
Sufficient For:
1000 Dual Luciferase Assays
Content And Storage:
Sufficient For: 1000 dual-luciferase assays in microplate wells• Renilla-Firefly Dual Assay Buffer, 50 mL (store at 4°C)• 100X Coelenterazine, 0.5 mL (store at -80°C)• D-Luciferin, 30 mg (store at 4°C)• Luciferase Cell Lysis Buffer (2X), 60 mL (store at room temperature)Upon receipt, store kit at -80°C, or store individual components as indicated above.
Compatible Cells:
Mammalian Cells
For Use With (Equipment):
Luminometer (Microplate)
Label Type:
Enzyme Labeled
Technique:
Enhanced Chemiluminescence
Product Line:
Pierce™
Target:
Luciferase, Firefly Luciferase, Green Renilla Luciferase
Quantity:
1000 Reaction Kit
Assay:
Reporter Enzyme, Luciferase Reporter Assay
Detection Method:
Bioluminescence
Format:
384-well plate, 96-well plate
Substrate Properties:
Chemical Substrate
Substrate Type:
Luciferase Substrate
Substrate:
Coelenterazine, D-Luciferin
Type: Cell Lysis Buffer
Sufficient For: __
Content And Storage: Store at room temperature.
Compatible Cells: __
For Use With (Equipment): __
Label Type: __
Technique: __
Product Line: __
Target: __
Quantity: 250 mL
Assay: __
Detection Method: __
Format: Liquid
Substrate Properties: __
Substrate Type: __
Substrate: __
Type:
Cell Lysis Buffer
Sufficient For:
__
Content And Storage:
Store at room temperature.
Compatible Cells:
__
For Use With (Equipment):
__
Label Type:
__
Technique:
__
Product Line:
__
Target:
__
Quantity:
250 mL
Assay:
__
Detection Method:
__
Format:
Liquid
Substrate Properties:
__
Substrate Type:
__
Substrate:
__
Type: Luc Vector
Sufficient For: __
Content And Storage: Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.
Compatible Cells: __
For Use With (Equipment): __
Label Type: __
Technique: __
Product Line: __
Target: __
Quantity: 10 μg
Assay: __
Detection Method: __
Format: __
Substrate Properties: __
Substrate Type: __
Substrate: __
Type:
Luc Vector
Sufficient For:
__
Content And Storage:
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: -70 to -20°C.
Compatible Cells:
__
For Use With (Equipment):
__
Label Type:
__
Technique:
__
Product Line:
__
Target:
__
Quantity:
10 μg
Assay:
__
Detection Method:
__
Format:
__
Substrate Properties:
__
Substrate Type:
__
Substrate:
__