PI16176
Thermo Scientific™ Pierce™ Firefly Luciferase Glow Assay Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI16176-Each-of-1 | In Stock | ₹ 14,613.65 |
PI16176 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 14,613.65
GST (18%): ₹ 2,630.457
Total Price: ₹ 17,244.107
Type
Assay Kit
Quantity
100 Reaction Kit
Assay
Reporter Enzyme, Luciferase Reporter Assay
Detection Method
Bioluminescence
Format
384-well plate, 96-well plate
Substrate Properties
Chemical Substrate
Substrate Type
Luciferase Substrate
Target
Luciferase, Firefly Luciferase
Content And Storage
• Firefly Glow Assay Buffer, 5 mL, store at -20°C• D-Luciferin, Lyophilized, 3 mg, store at 4°C• 2X Cell Lysis Buffer, 6 mL, store at room temperatureUpon receipt store kit at -20°C or store individual components as indicated above.
Compatible Cells
Mammalian Cells
For Use With (Equipment)
Luminometer (Microplate)
Label Type
Enzyme Labeled
Technique
Enhanced Chemiluminescence
Substrate
D-Luciferin
Related Products
Description
- The Thermo Scientific™ Pierce Firefly Luciferase Glow Assay Kit provides a bright and stable bioluminescence signal (half-life greater than two hours) in the presence of firefly luciferase reporters for protein expression assays
- This Pierce Luciferase Glow Assay Kit contains reagents for measuring the activity of firefly luciferase in cell lysates
- In the presence of firefly luciferase, the glow reagent provides stable bioluminescent signal and is highly suited for luminometers without injectors or for batch processing of samples
- The light output generated by the luciferase reaction can be correlated with the amount of luciferase protein produced which in turn is proportional to the promoter activity driving the luciferase expression
- The glow assay reagents were optimized for best results with Thermo Scientific Firefly Luc Plasmids; however, this assay kit may be used to detect the activity of other firefly or ATP-dependent luciferases that use D-luciferin as a substrate
- Highlights: Sensitive – highly sensitive detection of firefly luciferase activity; for best results (maximum signal) use with Thermo Scientific Pierce Firefly Luciferase Signal Enhancer Stable – increased signal stability compared to flash assays Simple – does not require a luminometer with injectors Compatible – assay reagents compatible with other firefly beetle luciferases Automation-friendly – amenable to high throughput assays Convenient – contains a universal cell lysis buffer and optimized glow assay reagent Safe – allows one to perform non-radioactive assays Includes: Cell lysis buffer, reaction buffer and substrate Requires: Firefly luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers; For best results (maximum signal), use with Thermo Scientific Pierce Firefly Luciferase Signal Enhancer Recommended for: Promoter studies for analyzing cis - regulatory elements and trans -acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies
Compare Similar Items
Show Difference
Type: Assay Kit
Quantity: 100 Reaction Kit
Assay: Reporter Enzyme, Luciferase Reporter Assay
Detection Method: Bioluminescence
Format: 384-well plate, 96-well plate
Substrate Properties: Chemical Substrate
Substrate Type: Luciferase Substrate
Target: Luciferase, Firefly Luciferase
Content And Storage: • Firefly Glow Assay Buffer, 5 mL, store at -20°C• D-Luciferin, Lyophilized, 3 mg, store at 4°C• 2X Cell Lysis Buffer, 6 mL, store at room temperatureUpon receipt store kit at -20°C or store individual components as indicated above.
Compatible Cells: Mammalian Cells
For Use With (Equipment): Luminometer (Microplate)
Label Type: Enzyme Labeled
Technique: Enhanced Chemiluminescence
Substrate: D-Luciferin
Type:
Assay Kit
Quantity:
100 Reaction Kit
Assay:
Reporter Enzyme, Luciferase Reporter Assay
Detection Method:
Bioluminescence
Format:
384-well plate, 96-well plate
Substrate Properties:
Chemical Substrate
Substrate Type:
Luciferase Substrate
Target:
Luciferase, Firefly Luciferase
Content And Storage:
• Firefly Glow Assay Buffer, 5 mL, store at -20°C• D-Luciferin, Lyophilized, 3 mg, store at 4°C• 2X Cell Lysis Buffer, 6 mL, store at room temperatureUpon receipt store kit at -20°C or store individual components as indicated above.
Compatible Cells:
Mammalian Cells
For Use With (Equipment):
Luminometer (Microplate)
Label Type:
Enzyme Labeled
Technique:
Enhanced Chemiluminescence
Substrate:
D-Luciferin
Type: Assay Kit
Quantity: 1000 Reaction Kit
Assay: Reporter Enzyme, Luciferase Reporter Assay
Detection Method: Bioluminescence
Format: 384-well plate, 96-well plate
Substrate Properties: Chemical Substrate
Substrate Type: Luciferase Substrate
Target: Luciferase, Firefly Luciferase
Content And Storage: • Firefly Glow Assay Buffer, 50 mL, store at -20°C• D-Luciferin, Lyophilized, 30 mg, store at 4°C• 2X Cell Lysis Buffer, 60 mL, store at room temperatureUpon receipt store kit at -20°C or store individual components as indicated above.
Compatible Cells: Mammalian Cells
For Use With (Equipment): Luminometer (Microplate)
Label Type: Enzyme Labeled
Technique: Enhanced Chemiluminescence
Substrate: D-Luciferin
Type:
Assay Kit
Quantity:
1000 Reaction Kit
Assay:
Reporter Enzyme, Luciferase Reporter Assay
Detection Method:
Bioluminescence
Format:
384-well plate, 96-well plate
Substrate Properties:
Chemical Substrate
Substrate Type:
Luciferase Substrate
Target:
Luciferase, Firefly Luciferase
Content And Storage:
• Firefly Glow Assay Buffer, 50 mL, store at -20°C• D-Luciferin, Lyophilized, 30 mg, store at 4°C• 2X Cell Lysis Buffer, 60 mL, store at room temperatureUpon receipt store kit at -20°C or store individual components as indicated above.
Compatible Cells:
Mammalian Cells
For Use With (Equipment):
Luminometer (Microplate)
Label Type:
Enzyme Labeled
Technique:
Enhanced Chemiluminescence
Substrate:
D-Luciferin
Type: Firefly Signal Enhancer
Quantity: 0.5 mL
Assay: __
Detection Method: Bioluminescence
Format: __
Substrate Properties: Chemical Substrate
Substrate Type: Luciferase Substrate
Target: __
Content And Storage: Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, but do not store above -20°C.
Compatible Cells: __
For Use With (Equipment): __
Label Type: __
Technique: __
Substrate: __
Type:
Firefly Signal Enhancer
Quantity:
0.5 mL
Assay:
__
Detection Method:
Bioluminescence
Format:
__
Substrate Properties:
Chemical Substrate
Substrate Type:
Luciferase Substrate
Target:
__
Content And Storage:
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, but do not store above -20°C.
Compatible Cells:
__
For Use With (Equipment):
__
Label Type:
__
Technique:
__
Substrate:
__
Type: Assay Kit
Quantity: 100 Reaction Kit
Assay: Reporter Enzyme, Luciferase Reporter Assay
Detection Method: Bioluminescence
Format: 384-well plate, 96-well plate
Substrate Properties: Chemical Substrate
Substrate Type: Luciferase Substrate
Target: Luciferase, Firefly Luciferase, Gaussia Luciferase
Content And Storage: • Gaussia-Firefly Dual Assay Buffer, 5 mL, store at 4°C• Coelenterazine (100X), 50 μL, store at -80°C• D-Luciferin, Lyophilized, 3 mg, store at 4°C• 2X Cell Lysis Buffer, 6 mL, store at room temperatureUpon receipt store kit at -80°C or store individual components as indicated above.
Compatible Cells: Mammalian Cells
For Use With (Equipment): Luminometer (Microplate)
Label Type: Enzyme Labeled
Technique: Enhanced Chemiluminescence
Substrate: Coelenterazine, D-Luciferin
Type:
Assay Kit
Quantity:
100 Reaction Kit
Assay:
Reporter Enzyme, Luciferase Reporter Assay
Detection Method:
Bioluminescence
Format:
384-well plate, 96-well plate
Substrate Properties:
Chemical Substrate
Substrate Type:
Luciferase Substrate
Target:
Luciferase, Firefly Luciferase, Gaussia Luciferase
Content And Storage:
• Gaussia-Firefly Dual Assay Buffer, 5 mL, store at 4°C• Coelenterazine (100X), 50 μL, store at -80°C• D-Luciferin, Lyophilized, 3 mg, store at 4°C• 2X Cell Lysis Buffer, 6 mL, store at room temperatureUpon receipt store kit at -80°C or store individual components as indicated above.
Compatible Cells:
Mammalian Cells
For Use With (Equipment):
Luminometer (Microplate)
Label Type:
Enzyme Labeled
Technique:
Enhanced Chemiluminescence
Substrate:
Coelenterazine, D-Luciferin