PI16175
Thermo Scientific™ Pierce™ Firefly Luciferase Flash Assay Kit
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI16175-Each-of-1 | In Stock | ₹ 71,613.72 |
PI16175 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 71,613.72
GST (18%): ₹ 12,890.47
Total Price: ₹ 84,504.19
Type
Assay Kit
Quantity
1000 Reaction Kit
Assay
Reporter Enzyme, Luciferase Reporter Assay
Detection Method
Bioluminescence
Format
384-well plate, 96-well plate
Substrate Properties
Chemical Substrate
Substrate Type
Luciferase Substrate
Substrate
D-Luciferin
Target
Luciferase, Firefly Luciferase
Content And Storage
• Firefly Flash Assay Buffer, 5 mL, store at 4°C• D-Luciferin, 3 mg, store at 4°C• 2X Cell Lysis Buffer, 6 mL, store at room temperatureUpon receipt store kit at 4°C or store individual components as indicated above.
Compatible Cells
Mammalian Cells
For Use With (Equipment)
Luminometer (Microplate)
Label Type
Enzyme Labeled
Technique
Enhanced Chemiluminescence
Product Line
Pierce™
Related Products
Description
- The Thermo Scientific™ Pierce Firefly Luciferase Flash Assay Kit provides reagents to measure Red firefly luciferase activity in whole cell producing a very strong flash signal which decays rapidly
- Thermo Scientific Pierce Firefly Luciferase Flash Assay Kit contains reagents to measure the activity of firefly luciferase in mammalian whole cell lysates
- Containing a convenient lysis buffer, assay buffer and firefly substrate, this kit provides a complete reagent system for the intracellular detection of promoter or pathway activity
- Light output resulting from the luciferase reaction is captured using a luminometer and can be correlated with the amount of firefly luciferase protein produced and used to determine the activity of the promoter driving firefly expression
- These reagents are optimized to work with Thermo Scientific Firefly Luc Plasmids; however, the Flash Assay Kit may be used to detect the activity of other firefly or ATP-dependent luciferases utilizing D-luciferin as a substrate
- Highlights: Highly sensitive detection of firefly luciferase activity Use with Thermo Scientific Pierce Firefly Luciferase Signal Enhancer for best results (maximum signal) Working solution stable for up to 2 months at -20°C Assay reagents compatible with other firefly luciferases Amenable to automation Contains a universal cell lysis buffer and optimized flash assay reagent Safe non-radioactive assay Includes: Cell lysis buffer, reaction buffer and substrate Requires: Firefly luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers; For best results (maximum signal), use with Thermo Scientific Pierce Firefly Luciferase Signal Enhancer Recommended for: Promoter studies for analyzing cis - regulatory elements and trans -acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Secretory pathway / protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies
Compare Similar Items
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Type: Assay Kit
Quantity: 1000 Reaction Kit
Assay: Reporter Enzyme, Luciferase Reporter Assay
Detection Method: Bioluminescence
Format: 384-well plate, 96-well plate
Substrate Properties: Chemical Substrate
Substrate Type: Luciferase Substrate
Substrate: D-Luciferin
Target: Luciferase, Firefly Luciferase
Content And Storage: • Firefly Flash Assay Buffer, 5 mL, store at 4°C• D-Luciferin, 3 mg, store at 4°C• 2X Cell Lysis Buffer, 6 mL, store at room temperatureUpon receipt store kit at 4°C or store individual components as indicated above.
Compatible Cells: Mammalian Cells
For Use With (Equipment): Luminometer (Microplate)
Label Type: Enzyme Labeled
Technique: Enhanced Chemiluminescence
Product Line: Pierce™
Type:
Assay Kit
Quantity:
1000 Reaction Kit
Assay:
Reporter Enzyme, Luciferase Reporter Assay
Detection Method:
Bioluminescence
Format:
384-well plate, 96-well plate
Substrate Properties:
Chemical Substrate
Substrate Type:
Luciferase Substrate
Substrate:
D-Luciferin
Target:
Luciferase, Firefly Luciferase
Content And Storage:
• Firefly Flash Assay Buffer, 5 mL, store at 4°C• D-Luciferin, 3 mg, store at 4°C• 2X Cell Lysis Buffer, 6 mL, store at room temperatureUpon receipt store kit at 4°C or store individual components as indicated above.
Compatible Cells:
Mammalian Cells
For Use With (Equipment):
Luminometer (Microplate)
Label Type:
Enzyme Labeled
Technique:
Enhanced Chemiluminescence
Product Line:
Pierce™
Type: Assay Kit
Quantity: 100 Reaction Kit
Assay: Reporter Enzyme, Luciferase Reporter Assay
Detection Method: Bioluminescence
Format: 384-well plate, 96-well plate
Substrate Properties: Chemical Substrate
Substrate Type: Luciferase Substrate
Substrate: D-Luciferin
Target: Luciferase, Firefly Luciferase
Content And Storage: • Firefly Glow Assay Buffer, 5 mL, store at -20°C• D-Luciferin, Lyophilized, 3 mg, store at 4°C• 2X Cell Lysis Buffer, 6 mL, store at room temperatureUpon receipt store kit at -20°C or store individual components as indicated above.
Compatible Cells: Mammalian Cells
For Use With (Equipment): Luminometer (Microplate)
Label Type: Enzyme Labeled
Technique: Enhanced Chemiluminescence
Product Line: __
Type:
Assay Kit
Quantity:
100 Reaction Kit
Assay:
Reporter Enzyme, Luciferase Reporter Assay
Detection Method:
Bioluminescence
Format:
384-well plate, 96-well plate
Substrate Properties:
Chemical Substrate
Substrate Type:
Luciferase Substrate
Substrate:
D-Luciferin
Target:
Luciferase, Firefly Luciferase
Content And Storage:
• Firefly Glow Assay Buffer, 5 mL, store at -20°C• D-Luciferin, Lyophilized, 3 mg, store at 4°C• 2X Cell Lysis Buffer, 6 mL, store at room temperatureUpon receipt store kit at -20°C or store individual components as indicated above.
Compatible Cells:
Mammalian Cells
For Use With (Equipment):
Luminometer (Microplate)
Label Type:
Enzyme Labeled
Technique:
Enhanced Chemiluminescence
Product Line:
__
Type: Assay Kit
Quantity: 1000 Reaction Kit
Assay: Reporter Enzyme, Luciferase Reporter Assay
Detection Method: Bioluminescence
Format: 384-well plate, 96-well plate
Substrate Properties: Chemical Substrate
Substrate Type: Luciferase Substrate
Substrate: D-Luciferin
Target: Luciferase, Firefly Luciferase
Content And Storage: • Firefly Glow Assay Buffer, 50 mL, store at -20°C• D-Luciferin, Lyophilized, 30 mg, store at 4°C• 2X Cell Lysis Buffer, 60 mL, store at room temperatureUpon receipt store kit at -20°C or store individual components as indicated above.
Compatible Cells: Mammalian Cells
For Use With (Equipment): Luminometer (Microplate)
Label Type: Enzyme Labeled
Technique: Enhanced Chemiluminescence
Product Line: Pierce™
Type:
Assay Kit
Quantity:
1000 Reaction Kit
Assay:
Reporter Enzyme, Luciferase Reporter Assay
Detection Method:
Bioluminescence
Format:
384-well plate, 96-well plate
Substrate Properties:
Chemical Substrate
Substrate Type:
Luciferase Substrate
Substrate:
D-Luciferin
Target:
Luciferase, Firefly Luciferase
Content And Storage:
• Firefly Glow Assay Buffer, 50 mL, store at -20°C• D-Luciferin, Lyophilized, 30 mg, store at 4°C• 2X Cell Lysis Buffer, 60 mL, store at room temperatureUpon receipt store kit at -20°C or store individual components as indicated above.
Compatible Cells:
Mammalian Cells
For Use With (Equipment):
Luminometer (Microplate)
Label Type:
Enzyme Labeled
Technique:
Enhanced Chemiluminescence
Product Line:
Pierce™
Type: Firefly Signal Enhancer
Quantity: 0.5 mL
Assay: __
Detection Method: Bioluminescence
Format: __
Substrate Properties: Chemical Substrate
Substrate Type: Luciferase Substrate
Substrate: __
Target: __
Content And Storage: Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, but do not store above -20°C.
Compatible Cells: __
For Use With (Equipment): __
Label Type: __
Technique: __
Product Line: Pierce™
Type:
Firefly Signal Enhancer
Quantity:
0.5 mL
Assay:
__
Detection Method:
Bioluminescence
Format:
__
Substrate Properties:
Chemical Substrate
Substrate Type:
Luciferase Substrate
Substrate:
__
Target:
__
Content And Storage:
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, but do not store above -20°C.
Compatible Cells:
__
For Use With (Equipment):
__
Label Type:
__
Technique:
__
Product Line:
Pierce™