PI16169
Thermo Scientific™ Pierce™ Cypridina Luciferase Flash Assay Kit
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI16169-Each-of-1 | In Stock | ₹ 66,266.22 |
PI16169 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 66,266.22
GST (18%): ₹ 11,927.92
Total Price: ₹ 78,194.14
Type
Assay Kit
Quantity
1000 Reaction Kit
Assay
Reporter Enzyme, Luciferase Reporter Assay
Detection Method
Bioluminescence
Format
384-well plate, 96-well plate
Substrate Properties
Chemical Substrate
Substrate Type
Luciferase Substrate
Substrate
Vargulin (Cypridina Luciferin)
Target
Luciferase, Cypridina Luciferase
Content And Storage
• Cypridina Flash Assay Buffer, 5 mL, store at 4°C• Vargulin (1X), 0.5 mL, store at -20°C• 2X Cell Lysis Buffer, 6 mL, store at room temperatureUpon receipt store kit at -20°C or store individual components as indicated above.
Compatible Cells
Mammalian Cells
For Use With (Equipment)
Luminometer (Microplate)
Label Type
Enzyme Labeled
Technique
Enhanced Chemiluminescence
Product Line
Pierce™
Related Products
Description
- The Thermo Scientific™ Pierce Cypridina Luciferase Flash Assay Kit provides researchers with a highly sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate
- This Flash Assay Kit contains reagents for measuring the activity of Cypridina luciferase in mammalian cell culture media and lysates
- When used with Thermo Scientific Cypridina Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of promoter or pathway activity
- Cypridina luciferase is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity
- The signal produced by Cypridina is considerably greater than signal from either firefly or Renilla luciferases assayed under similar conditions
- Highlights: Sensitive – high sensitivity allows utilization of smaller numbers of cells Cost effective – sensitive assays result in decreased reagent consumption Secreted luciferase – allows real-time assays and kinetic studies without destroying cells Ideal for co-transfection – uses a different substrate than Renilla , Gaussia , or firefly luciferases Compatible – assay reagents compatible with other Cypridina or Vargula luciferases Time-saving – assays using secreted luciferases require minimal sample handling Automation-friendly – amenable to high throughput screens Convenient – contains a universal cell lysis buffer and optimized flash assay reagent Safe – allows one to perform non-radioactive assays Includes: Cell lysis buffer, reaction buffer and substrate Requires: Cypridina luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers
- Recommended for: Promoter studies for analyzing cis - regulatory elements and trans -acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Secretory pathway / protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies
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Type: Assay Kit
Quantity: 1000 Reaction Kit
Assay: Reporter Enzyme, Luciferase Reporter Assay
Detection Method: Bioluminescence
Format: 384-well plate, 96-well plate
Substrate Properties: Chemical Substrate
Substrate Type: Luciferase Substrate
Substrate: Vargulin (Cypridina Luciferin)
Target: Luciferase, Cypridina Luciferase
Content And Storage: • Cypridina Flash Assay Buffer, 5 mL, store at 4°C• Vargulin (1X), 0.5 mL, store at -20°C• 2X Cell Lysis Buffer, 6 mL, store at room temperatureUpon receipt store kit at -20°C or store individual components as indicated above.
Compatible Cells: Mammalian Cells
For Use With (Equipment): Luminometer (Microplate)
Label Type: Enzyme Labeled
Technique: Enhanced Chemiluminescence
Product Line: Pierce™
Type:
Assay Kit
Quantity:
1000 Reaction Kit
Assay:
Reporter Enzyme, Luciferase Reporter Assay
Detection Method:
Bioluminescence
Format:
384-well plate, 96-well plate
Substrate Properties:
Chemical Substrate
Substrate Type:
Luciferase Substrate
Substrate:
Vargulin (Cypridina Luciferin)
Target:
Luciferase, Cypridina Luciferase
Content And Storage:
• Cypridina Flash Assay Buffer, 5 mL, store at 4°C• Vargulin (1X), 0.5 mL, store at -20°C• 2X Cell Lysis Buffer, 6 mL, store at room temperatureUpon receipt store kit at -20°C or store individual components as indicated above.
Compatible Cells:
Mammalian Cells
For Use With (Equipment):
Luminometer (Microplate)
Label Type:
Enzyme Labeled
Technique:
Enhanced Chemiluminescence
Product Line:
Pierce™
Type: Assay Kit
Quantity: 100 Reaction Kit
Assay: Reporter Enzyme, Luciferase Reporter Assay
Detection Method: Bioluminescence
Format: 384-well plate, 96-well plate
Substrate Properties: Chemical Substrate
Substrate Type: Luciferase Substrate
Substrate: Vargulin (Cypridina Luciferin)
Target: Luciferase, Cypridina Luciferase
Content And Storage: • Cypridina Glow Assay Buffer, 5 mL, store at 4°C• Vargulin (1X), 5 μL, store at -20°C• 2X Cell Lysis Buffer, 6 mL, store at room temperatureUpon receipt store kit at -20°C or store individual components as indicated above.
Compatible Cells: Mammalian Cells
For Use With (Equipment): Luminometer (Microplate)
Label Type: Enzyme Labeled
Technique: Enhanced Chemiluminescence
Product Line: Pierce™
Type:
Assay Kit
Quantity:
100 Reaction Kit
Assay:
Reporter Enzyme, Luciferase Reporter Assay
Detection Method:
Bioluminescence
Format:
384-well plate, 96-well plate
Substrate Properties:
Chemical Substrate
Substrate Type:
Luciferase Substrate
Substrate:
Vargulin (Cypridina Luciferin)
Target:
Luciferase, Cypridina Luciferase
Content And Storage:
• Cypridina Glow Assay Buffer, 5 mL, store at 4°C• Vargulin (1X), 5 μL, store at -20°C• 2X Cell Lysis Buffer, 6 mL, store at room temperatureUpon receipt store kit at -20°C or store individual components as indicated above.
Compatible Cells:
Mammalian Cells
For Use With (Equipment):
Luminometer (Microplate)
Label Type:
Enzyme Labeled
Technique:
Enhanced Chemiluminescence
Product Line:
Pierce™
Type: Assay Kit
Quantity: 1000 Reaction Kit
Assay: Reporter Enzyme, Luciferase Reporter Assay
Detection Method: Bioluminescence
Format: 384-well plate, 96-well plate
Substrate Properties: Chemical Substrate
Substrate Type: Luciferase Substrate
Substrate: Vargulin (Cypridina Luciferin)
Target: Luciferase, Cypridina Luciferase
Content And Storage: • Cypridina Glow Assay Buffer, 5 mL, store at 4°C• Vargulin (1X), 0.5 mL, store at -20°C• 2X Cell Lysis Buffer, 6 mL, store at room temperatureUpon receipt store kit at -20°C or store individual components as indicated above.
Compatible Cells: Mammalian Cells
For Use With (Equipment): Luminometer (Microplate)
Label Type: Enzyme Labeled
Technique: Enhanced Chemiluminescence
Product Line: Pierce™
Type:
Assay Kit
Quantity:
1000 Reaction Kit
Assay:
Reporter Enzyme, Luciferase Reporter Assay
Detection Method:
Bioluminescence
Format:
384-well plate, 96-well plate
Substrate Properties:
Chemical Substrate
Substrate Type:
Luciferase Substrate
Substrate:
Vargulin (Cypridina Luciferin)
Target:
Luciferase, Cypridina Luciferase
Content And Storage:
• Cypridina Glow Assay Buffer, 5 mL, store at 4°C• Vargulin (1X), 0.5 mL, store at -20°C• 2X Cell Lysis Buffer, 6 mL, store at room temperatureUpon receipt store kit at -20°C or store individual components as indicated above.
Compatible Cells:
Mammalian Cells
For Use With (Equipment):
Luminometer (Microplate)
Label Type:
Enzyme Labeled
Technique:
Enhanced Chemiluminescence
Product Line:
Pierce™
Type: Assay Kit
Quantity: 100 Reaction Kit
Assay: Reporter Enzyme, Luciferase Reporter Assay
Detection Method: Bioluminescence
Format: 384-well plate, 96-well plate
Substrate Properties: Chemical Substrate
Substrate Type: Luciferase Substrate
Substrate: D-Luciferin
Target: Luciferase, Firefly Luciferase
Content And Storage: • Firefly Flash Assay Buffer, 5 mL, store at 4°C• D-Luciferin, Lyophilized, 3 mg, store at 4°C• 2X Cell Lysis Buffer, 6 mL, store at room temperatureUpon receipt store kit at 4°C or store individual components as indicated above.
Compatible Cells: Mammalian Cells
For Use With (Equipment): Luminometer (Microplate)
Label Type: Enzyme Labeled
Technique: Enhanced Chemiluminescence
Product Line: Pierce™
Type:
Assay Kit
Quantity:
100 Reaction Kit
Assay:
Reporter Enzyme, Luciferase Reporter Assay
Detection Method:
Bioluminescence
Format:
384-well plate, 96-well plate
Substrate Properties:
Chemical Substrate
Substrate Type:
Luciferase Substrate
Substrate:
D-Luciferin
Target:
Luciferase, Firefly Luciferase
Content And Storage:
• Firefly Flash Assay Buffer, 5 mL, store at 4°C• D-Luciferin, Lyophilized, 3 mg, store at 4°C• 2X Cell Lysis Buffer, 6 mL, store at room temperatureUpon receipt store kit at 4°C or store individual components as indicated above.
Compatible Cells:
Mammalian Cells
For Use With (Equipment):
Luminometer (Microplate)
Label Type:
Enzyme Labeled
Technique:
Enhanced Chemiluminescence
Product Line:
Pierce™