PI23260
Thermo Scientific™ Pierce™ Glycoprotein Carbohydrate Estimation Kit
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI23260-Each-of-1 | In Stock | ₹ 53,303.88 |
PI23260 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 53,303.88
GST (18%): ₹ 9,594.698
Total Price: ₹ 62,898.578
Content And Storage
Sufficient For: 60 tube assays or 250 microplate assays• Sodium meta-Periodate, 500 mg• Pierce Glycoprotein Detection Reagent, 500 mg• Glycoprotein Assay Buffer, 250 mL• Glycoprotein Standards Set, 5-protein set
Assay
Glycoprotein Carbohydrate Estimation
Product Line
Pierce™
Specificity
Not Target-Specific
For Use With (Application)
Solution-based Detection, Absorbance
Quantity
500 mg
Description
Pierce Glycoprotein Carbohydrate Estimation Kit
Detection Method
Colorimetric
Product Type
Glycoprotein Carbohydrate Estimation
Sufficient For
60 Tube Assays or 250 Microplate Assays
For Use With (Equipment)
Spectrophotometer, Microplate Reader
Related Products
Description
- Thermo Scientific™ Pierce Glycoprotein Carbohydrate Estimation Kit enables the amount of protein glycosylation to be measured as the percent of total purified protein mass
- The Glycoprotein Carbohydrate Estimation Kit includes six purified glycoprotein standards and the required assay reagents to effectively estimate the amount of oxidizable glycosylation (% carbohydrate by weight) of purified protein samples
- Sugar groups in the glycoprotein sample are first oxidized with sodium meta-periodate to produce detectable aldehyde groups
- Then the sample is reacted with the colorimetric Glycoprotein Detection Reagent
- Absorbance at 550nm of the resulting purple reaction product is measured with a spectrophotometer or plate reader
- Finally, the carbohydrate content in the glycoprotein is calculated by comparison to results from the five glycoprotein standards that are included in the kit
- Highlights: Qualitative – easily identifies purified proteins as glycoproteins or samples as contaminated with sugars Semi-quantitative – estimates the percent carbohydrate content (w/w) of purified glycoprotein by comparison to the included set of glycoprotein standards Simple procedure – completed in less than 75 minutes; instructions include microplate and test tube protocols Adaptable – standard curve format allows for the design of alternative tests for aldehydes and carbohydrate components Includes: Sodium periodate, assay buffer, detection reagent and a 5-vial set of glycoprotein standards Recommended for: Determine if a purified polyclonal antibody or other protein is glycosylated before attempting to perform carbohydrate-based conjugation or immobilization chemistries with hydrazide reagents; Characterize and classify purified protein fractions from an affinity procedure; Assess sugar and carbohydrate contamination in non-glycosylated protein samples
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Content And Storage: Sufficient For: 60 tube assays or 250 microplate assays• Sodium meta-Periodate, 500 mg• Pierce Glycoprotein Detection Reagent, 500 mg• Glycoprotein Assay Buffer, 250 mL• Glycoprotein Standards Set, 5-protein set
Assay: Glycoprotein Carbohydrate Estimation
Product Line: Pierce™
Specificity: Not Target-Specific
For Use With (Application): Solution-based Detection, Absorbance
Quantity: 500 mg
Description: Pierce Glycoprotein Carbohydrate Estimation Kit
Detection Method: Colorimetric
Product Type: Glycoprotein Carbohydrate Estimation
Sufficient For: 60 Tube Assays or 250 Microplate Assays
For Use With (Equipment): Spectrophotometer, Microplate Reader
Content And Storage:
Sufficient For: 60 tube assays or 250 microplate assays• Sodium meta-Periodate, 500 mg• Pierce Glycoprotein Detection Reagent, 500 mg• Glycoprotein Assay Buffer, 250 mL• Glycoprotein Standards Set, 5-protein set
Assay:
Glycoprotein Carbohydrate Estimation
Product Line:
Pierce™
Specificity:
Not Target-Specific
For Use With (Application):
Solution-based Detection, Absorbance
Quantity:
500 mg
Description:
Pierce Glycoprotein Carbohydrate Estimation Kit
Detection Method:
Colorimetric
Product Type:
Glycoprotein Carbohydrate Estimation
Sufficient For:
60 Tube Assays or 250 Microplate Assays
For Use With (Equipment):
Spectrophotometer, Microplate Reader
Content And Storage: Sufficient For: 250 microplate assays using a standard plate reader (absorbance at 450 nm)• Succinylated Casein, 5 x 10 mg• TNBSA Solution, 2 mL• TPCK Trypsin Standard, 50 mg• Borate Buffer Pack (makes 500 mL), 1 packStore at 4°C.
Assay: Protease Assay
Product Line: Pierce™
Specificity: __
For Use With (Application): Absorbance
Quantity: 250 Test Kit
Description: __
Detection Method: Colorimetric
Product Type: Protein Activity Assay
Sufficient For: 250 Microplate Assays
For Use With (Equipment): Microplate Reader
Content And Storage:
Sufficient For: 250 microplate assays using a standard plate reader (absorbance at 450 nm)• Succinylated Casein, 5 x 10 mg• TNBSA Solution, 2 mL• TPCK Trypsin Standard, 50 mg• Borate Buffer Pack (makes 500 mL), 1 packStore at 4°C.
Assay:
Protease Assay
Product Line:
Pierce™
Specificity:
__
For Use With (Application):
Absorbance
Quantity:
250 Test Kit
Description:
__
Detection Method:
Colorimetric
Product Type:
Protein Activity Assay
Sufficient For:
250 Microplate Assays
For Use With (Equipment):
Microplate Reader
Content And Storage: Sufficient For: 2000 microplate assays using an instrument fitted with a fluorescein filter set (ex/em 485 nm/538 nm)• FTC-Casein, lyophilized, 2.5 mg• TPCK Trypsin Standard, 50 mg• TBS Buffer Pack (makes 500 mL), 1 packStore FTC-Casein and TPCK Trypsin at 4°C.
Assay: Protease Assay
Product Line: Pierce™
Specificity: __
For Use With (Application): Fluorescence Polarization, FRET
Quantity: 2000 Test Kit
Description: __
Detection Method: Fluorescence
Product Type: Protein Activity Assay
Sufficient For: 2000 Microplate Assays
For Use With (Equipment): Microplate Reader
Content And Storage:
Sufficient For: 2000 microplate assays using an instrument fitted with a fluorescein filter set (ex/em 485 nm/538 nm)• FTC-Casein, lyophilized, 2.5 mg• TPCK Trypsin Standard, 50 mg• TBS Buffer Pack (makes 500 mL), 1 packStore FTC-Casein and TPCK Trypsin at 4°C.
Assay:
Protease Assay
Product Line:
Pierce™
Specificity:
__
For Use With (Application):
Fluorescence Polarization, FRET
Quantity:
2000 Test Kit
Description:
__
Detection Method:
Fluorescence
Product Type:
Protein Activity Assay
Sufficient For:
2000 Microplate Assays
For Use With (Equipment):
Microplate Reader
Content And Storage: Upon receipt store FTC-Casein at 4°C. Product shipped at ambient temperature.
Assay: Substrate
Product Line: Pierce™
Specificity: __
For Use With (Application): Fluorescence Polarization, FRET
Quantity: 2.5 mg
Description: __
Detection Method: Fluorescence
Product Type: Protein Activity Assay
Sufficient For: __
For Use With (Equipment): Microplate Reader
Content And Storage:
Upon receipt store FTC-Casein at 4°C. Product shipped at ambient temperature.
Assay:
Substrate
Product Line:
Pierce™
Specificity:
__
For Use With (Application):
Fluorescence Polarization, FRET
Quantity:
2.5 mg
Description:
__
Detection Method:
Fluorescence
Product Type:
Protein Activity Assay
Sufficient For:
__
For Use With (Equipment):
Microplate Reader