PI23282
Thermo Scientific™ Pierce™ Peroxide Assay Reagent B
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI23282-Each-of-1 | In Stock | ₹ 14,863.00 |
PI23282 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 14,863.00
GST (18%): ₹ 2,675.34
Total Price: ₹ 17,538.34
Content And Storage
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 8°C.
Assay
Peroxide Assay Reagent B
Product Line
Pierce™
Specificity
Not Target-Specific
For Use With (Equipment)
Spectrophotometer, Microplate Reader
Description
Pierce Peroxide Assay Reagent B
Detection Method
Colorimetric
Product Type
Quantitative Peroxide Assay
For Use With (Application)
Solution-based Detection, Absorbance
Related Products
Description
- Detect and measure hydrogen peroxide levels in biological samples using an iron and xylenol orange reagent with Thermo Scientific™ Pierce Quantitative Peroxide Assay Kits
- These aqueous and lipid-compatible peroxide assay kits detect peroxides based on oxidation of ferrous to ferric ion in the presence of xylenol orange
- One assay kit has an aqueous-compatible formulation that includes sorbitol, which provides sensitivity enhancement
- The other assay kit has a formulation that may be used with lipid-containing samples without first extracting the lipids; however, because this kit does not contain sorbitol, the assay is less sensitive
- Most proteins do not interfere with these assays, although some metal chelators may require use of a blank (i.e., control) to mitigate these effects
- In these assays, hydroperoxides convert the Fe2+ to Fe3+ at acidic pH
- With the aqueous-compatible formulation, peroxide first reacts with sorbitol, converting it to a peroxyl radical, which in turn initiates Fe2+ oxidation to Fe3+
- In the lipid compatible formulation, the peroxide converts the Fe2+ to Fe3+ directly
- In a sulfuric acid solution, the Fe3+ complexes with the xylenol orange (XO) dye to yield a purple product having an absorbance maximum at 560nm
- Peroxide levels in test samples can be determined by calculation from the know extinction coefficient of the XO-Fe complex or by reference to a standard curve prepared with hydrogen peroxide solution
- Highlights: Peroxides – detects and measures hydrogen peroxide (H2O2) levels in biological and other liquids samples Convenient – no proprietary reagents involved but the ready-to-use kits remove the variability, difficulty and added expense of formulating the reagents yourself Versatile – quantitation by calculation from the extinction coefficient or by reference to a standard curve enables the method to be easily adapted to a variety of sample types and testing purposes Two formulations – choose the aqueous-compatible kit for maximum sensitivity with non-lipid samples; choose the lipid-compatible kit for best results with lipid-containing samples Recommended for: General hydrogen peroxide (H 2 O 2 ) measurement in protein samples; Quantitation of peroxides in detergents; Assessment of the effects of peroxides on cellular activity; Determination of protein glycation; Measurement of peroxide accumulation in lipids
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Content And Storage: Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 8°C.
Assay: Peroxide Assay Reagent B
Product Line: Pierce™
Specificity: Not Target-Specific
For Use With (Equipment): Spectrophotometer, Microplate Reader
Description: Pierce Peroxide Assay Reagent B
Detection Method: Colorimetric
Product Type: Quantitative Peroxide Assay
For Use With (Application): Solution-based Detection, Absorbance
Content And Storage:
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 8°C.
Assay:
Peroxide Assay Reagent B
Product Line:
Pierce™
Specificity:
Not Target-Specific
For Use With (Equipment):
Spectrophotometer, Microplate Reader
Description:
Pierce Peroxide Assay Reagent B
Detection Method:
Colorimetric
Product Type:
Quantitative Peroxide Assay
For Use With (Application):
Solution-based Detection, Absorbance
Content And Storage: Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 8°C.
Assay: Peroxide Assay Reagent C
Product Line: Pierce™
Specificity: Not Target-Specific
For Use With (Equipment): Spectrophotometer, Microplate Reader
Description: Pierce Peroxide Assay Reagent C
Detection Method: Colorimetric
Product Type: Quantitative Peroxide Assay
For Use With (Application): Solution-based Detection, Absorbance
Content And Storage:
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 8°C.
Assay:
Peroxide Assay Reagent C
Product Line:
Pierce™
Specificity:
Not Target-Specific
For Use With (Equipment):
Spectrophotometer, Microplate Reader
Description:
Pierce Peroxide Assay Reagent C
Detection Method:
Colorimetric
Product Type:
Quantitative Peroxide Assay
For Use With (Application):
Solution-based Detection, Absorbance
Content And Storage: Sufficient For: 100 tube assays or 500 microplate assays with lipid-containing samples• Reagent A, 1 mL• Reagent C, 4 x 25 mLStore at 4°C.
Assay: Quantitative Peroxide Assay Kit
Product Line: Pierce™
Specificity: Not Target-Specific
For Use With (Equipment): Spectrophotometer, Microplate Reader
Description: Pierce Quantitative Peroxide Assay Kit (Lipid)
Detection Method: Colorimetric
Product Type: Quantitative Peroxide Assay
For Use With (Application): Solution-based Detection, Absorbance
Content And Storage:
Sufficient For: 100 tube assays or 500 microplate assays with lipid-containing samples• Reagent A, 1 mL• Reagent C, 4 x 25 mLStore at 4°C.
Assay:
Quantitative Peroxide Assay Kit
Product Line:
Pierce™
Specificity:
Not Target-Specific
For Use With (Equipment):
Spectrophotometer, Microplate Reader
Description:
Pierce Quantitative Peroxide Assay Kit (Lipid)
Detection Method:
Colorimetric
Product Type:
Quantitative Peroxide Assay
For Use With (Application):
Solution-based Detection, Absorbance
Content And Storage: Fluorometric Peptide Assay Reagent, 4 x 2.5 mLFluorometric Peptide Assay Buffer, 50 mLPeptide Digest Assay Standard, 1.5 mLStore in refrigerator (2–8°C).
Assay: __
Product Line: Pierce™
Specificity: __
For Use With (Equipment): Microplate Reader
Description: Quantitative Fluorometric Peptide Assay
Detection Method: Fluorescence
Product Type: Peptide Assay
For Use With (Application): __
Content And Storage:
Fluorometric Peptide Assay Reagent, 4 x 2.5 mLFluorometric Peptide Assay Buffer, 50 mLPeptide Digest Assay Standard, 1.5 mLStore in refrigerator (2–8°C).
Assay:
__
Product Line:
Pierce™
Specificity:
__
For Use With (Equipment):
Microplate Reader
Description:
Quantitative Fluorometric Peptide Assay
Detection Method:
Fluorescence
Product Type:
Peptide Assay
For Use With (Application):
__