Invitrogen™ LanthaScreen™ TR-FRET BACE1 Assay Kit

Description

• BACE1 (beta-secretase) is a key enzyme involved in the production of amyloid beta-peptides found in extracellular amyloid plaques of Alzheimer's disease (AD)
• In some cases, early-onset familial AD can be attributed to a 'Swedish' mutation in the amyloid precursor protein (APP), which dramatically enhances the cleavage of this protein by BACE1
• This and other genetic and pathological evidence has led to therapeutic approaches that focus on the inhibition of BACE1 and other APP-cleaving enzymes, such as gamma-secretase
• Invitrogen's LanthaScreen™ TR-FRET BACE1 assay provides sensitive high-throughput screening for potential inhibitors of beta-secretase
• The kit uses a terbium (Tb)-labeled anti-biotin antibody and a fluorescein-labeled BACE1-biotin substrate in a homogeneous TR-FRET assay format (Figure 1)
• Contents and Storage: The LanthaScreen™ TR-FRET BACE1 Assay Kit contains BACE1 protein, fluorescently labeled BACE1 substrate, Tb-labeled anti-biotin antibody, and buffers
• Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).