PI31489

Thermo Scientific™ EZ-Link™ Plus Activated Peroxidase Kit

Manufacturer: Thermo Scientific™

Select a Size

Pack Size SKU Availability Price
Pack of 1 PI31489-Pack-of-1 In Stock ₹ 50,138.16

PI31489 - Pack of 1

₹ 50,138.16

In Stock

Quantity

1

Base Price: ₹ 50,138.16

GST (18%): ₹ 9,024.869

Total Price: ₹ 59,163.029

Quantity

1-plate kit

Product Line

EZ-Link™

Labeling Target

Antibodies (General), Proteins (General)

Labeling Scale

0.5-1 mg

Content And Storage

Sufficient For: 5 enzyme-labeling reactions, each with up to 1 mg of antibody (IgG)• Plus Activated Peroxidase, 5 x 1 mg• Sodium cyanoborohydride solution, 1 x 0.5 mL• Quenching Buffer, 25 mL• PBS Pack (makes 500 mL), 1 pack• Carbonate-Bicarbonate Buffer Pack (makes 500 mL), 1 packStore at -20°C.

Sufficient For

5 Enzyme Labeling Reactions

Label or Dye

HRP (Horseradish Peroxidase)

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Description

  • Thermo Scientific™ EZ-Link™ Plus Activated Peroxidase Kit contains horseradish peroxidase enzyme that has been aldehyde-activated with periodate to enable high-efficiency labeling and preparation of protein or antibody conjugates
  • Other amine-reactive chemistries, like glutaraldehyde, have a tendency to polymerize and produce lower amounts of viable conjugate
  • Sugar residues present on HRP have been oxidized with periodate to produce aldehydes that react with primary amines
  • Plus Activated Peroxidase is mixed with the protein to be coupled at a pH compatible with your protein or antibody
  • After incubating the reaction for one to two hours, the conjugate linkage is reduced and the activated HRP quenched with ethanolamine
  • The conjugate is purified by desalting or by dialysis
  • If you are preparing an antibody-HRP conjugate, use the Thermo Scientific Pierce Conjugate Purification Kit (Part No
  • 44920) to separate unreacted enzyme from the conjugate
  • Highlights: Reacts with readily accessible primary amines to form a covalent secondary amine bond Can be stored for at least 12 months at -20°C Consistent conjugation yields reliable conjugates Enzyme activity is 120-200 units/mg

Compare Similar Items

Show Difference

Img

Thermo Scientific™

PI31489

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Quantity:
1-plate kit

Product Line:
EZ-Link™

Labeling Target:
Antibodies (General), Proteins (General)

Labeling Scale:
0.5-1 mg

Content And Storage:
Sufficient For: 5 enzyme-labeling reactions, each with up to 1 mg of antibody (IgG)• Plus Activated Peroxidase, 5 x 1 mg• Sodium cyanoborohydride solution, 1 x 0.5 mL• Quenching Buffer, 25 mL• PBS Pack (makes 500 mL), 1 pack• Carbonate-Bicarbonate Buffer Pack (makes 500 mL), 1 packStore at -20°C.

Sufficient For:
5 Enzyme Labeling Reactions

Label or Dye:
HRP (Horseradish Peroxidase)

Img

Fischer Scientific

PI31490

--


Quantity:
10 mg

Product Line:
Pierce™

Labeling Target:
__

Labeling Scale:
__

Content And Storage:
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, but do not store above -20°C.

Sufficient For:
__

Label or Dye:
HRP (Horseradish Peroxidase)

Img

Fischer Scientific

PI31491

--


Quantity:
100 mg

Product Line:
Pierce™

Labeling Target:
__

Labeling Scale:
__

Content And Storage:
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, but do not store above -20°C.

Sufficient For:
__

Label or Dye:
HRP (Horseradish Peroxidase)

Img

Thermo Scientific™

PI31494

--


Quantity:
1 Reaction

Product Line:
EZ-Link™

Labeling Target:
__

Labeling Scale:
__

Content And Storage:
Sufficient For: 1 enzyme-labeling reaction with up to 5 mg of antibody (IgG)• Maleimide Activated Horseradish Peroxidase, 5 mg• Conjugation Buffer (10X), 20 mL• PBS Pack (makes 500mL), 1 pack• 2-Mercaptoethylamine-HCl, 6 mg• SATA, 2 mg• Dimethylformamide, 1 mL• Hydroxylamine-HCl, 5 mg• Polyacrylamide Desalting Column, 10 mL, 1 columnStore at 4°C.

Sufficient For:
1 Enzyme Labeling Reaction

Label or Dye:
HRP (Horseradish Peroxidase)