Thermo Scientific™ Pierce™ MS-Compatible Magnetic IP Kits

Description

• Immunoprecipitation is often necessary for the detection and quantitation of low-abundance proteins or rare signaling events
• Mass spectrometry analysis relies on two methods for the detection of low-abundance proteins: single reaction monitoring (SRM) and parallel reaction monitoring (PRM), which enable isolation, scheduling, and quantitation of selected proteotypic peptides
• These IP kits have been optimized for efficient immunoprecipitation and co-immunoprecipitation of target proteins, and are directly compatible with downstream MS sample preparation and analysis
• • MS-compatible —reagents directly compatible with in-solution peptide digestion • Sensitive —procedure successfully enriches low-abundance proteins (low ng range) • Low background —enriched samples contain minimal detergent residuals • Reduced antibody contamination —antibody biotinylation with streptavidin enrichment reduces contamination in the eluate when compared to other IP methods • Robust —procedure and reagents have been verified with numerous targets to ensure consistent enrichment of low-abundance proteins (ng range) with at least two peptides identified per protein The Thermo Scientific Pierce MS-Compatible Magnetic IP Kit (Protein A/G) uses high-quality Pierce Protein A/G Magnetic Beads to provide wider flexibility of antibody capture than either Protein A or G alone
• The Thermo Scientific Pierce MS-Compatible Magnetic IP Kit (Streptavidin) uses high-quality Pierce Streptavidin Magnetic Beads for use with biotinylated antibodies that may be purchased commercially or labeled using the Pierce Antibody Biotinylation Kit for IP
• After the immunoprecipitation procedure, the target-enriched elution fraction is ready for in-solution tryptic digestion for LC-MS analysis without the need for gel purification, detergent removal, or desalting
• Additionally, the reagents and procedures have been validated using both manual and automated magnetic separation
• Applications • Enrichment of low-abundance targets to improve MS sensitivity • Identification of target proteins that are not detectable by western blotting • Quantitation of target proteins by LC-MS analysis • Screening of antibodies for IP-MS