FERA51743
Thermo Scientific™ SureQuant™ IP-MS Sample Preparation Kits
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | FERA51743-Each-of-1 | In Stock | ₹ 54,843.96 |
FERA51743 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 54,843.96
GST (18%): ₹ 9,871.913
Total Price: ₹ 64,715.873
Description
Protein A/G Magnetic Beads
For Use With (Equipment)
Mass spectrometer
Content And Storage
Store trypsin at 4°C.Store trypsin storage solution at -20°C.
Detection Method
Mass Spectrometry
Quantity
20 Reactions
Kit Contents
Trypsin Protease, 2 x 20 μgTrypsin Storage Solution, 250 μLProtein A/G Magnetic Beads, 1 mLIP-MS Cell Lysis Buffer, 100 mLIP-MS Wash Buffer A, 75 mLIP-MS Wash Buffer B, 40 mLIP Elution and MS Sample Prep Buffer, 10 mLAlkylation Solution, 1 mL/vialLow Protein-Binding Collection Tubes, 50 x 1.5 mLBond-Breaker™ TCEP Solution, Neutral pH, 0.5 mL10% Pierce™ Trifluoroacetic Acid (TFA), Sequencing Grade, 0.5 mL1M MgCl2, 0.75 mL
Product Line
SureQuant™
Starting Material
Cells, Tissue
Workflow Step
Sample prep enrichment
For Use With (Application)
MS-compatible immunoprecipitation
Related Products
Description
- Thermo Scientific SureQuant IP-MS Sample Preparation kits (Protein A/G or streptavidin) provide an optimized solution for the immunoprecipitation and MS peptide preparation of samples upstream of LC-MS/MS analysis
- These kits have been rigorously verified using numerous multiplexed target antigens with varying expression levels
- The SureQuant product family enables the use of the immunoprecipitation (IP) sample prep kits and peptide quantitation modules together or independently for SRM/PRM analysis
- • Complete —kits include reagents for successful immunoprecipitation and MS peptide preparation for target proteins for SRM/PRM analysis • Multiplex —IP-MS kits have been designed for multiplex IP using both Protein A/G and Streptavidin magnetic beads • Flexible —universal reagents enable individualized selection of targets for IP to be used alone or combined with SureQuant peptide quantitation panels • Optimized —simplified and condensed procedure to prepare samples for LC-MS analysis • Low background —binding, wash, and elution buffers optimized to remove contaminants and minimize enrichment of background proteins The SureQuant Protein A/G and Streptavidin IP-MS Sample Preparation kits have been designed to support targeted quantitation using SureQuant or SRM/PRM analysis
- The kits contain high-quality Pierce Protein A/G or Streptavidin magnetic beads together with reagents that have been optimized for MS compatibility
- The streamlined procedure enables digestion immediately after the immunoprecipitation elution step, facilitating MS sample preparation in ∼4 hours for same-day LC-MS/MS analysis
- Applications: • Enrichment of low abundance targets to improve MS sensitivity • Identification of target proteins that are not detectable by western blotting • Quantitation of target proteins by LC-MS analysis • Screening of antibodies for IP-MS • Enrichment of target proteins upstream of targeted analysis (SRM)
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Description: Protein A/G Magnetic Beads
For Use With (Equipment): Mass spectrometer
Content And Storage: Store trypsin at 4°C.Store trypsin storage solution at -20°C.
Detection Method: Mass Spectrometry
Quantity: 20 Reactions
Kit Contents: Trypsin Protease, 2 x 20 μgTrypsin Storage Solution, 250 μLProtein A/G Magnetic Beads, 1 mLIP-MS Cell Lysis Buffer, 100 mLIP-MS Wash Buffer A, 75 mLIP-MS Wash Buffer B, 40 mLIP Elution and MS Sample Prep Buffer, 10 mLAlkylation Solution, 1 mL/vialLow Protein-Binding Collection Tubes, 50 x 1.5 mLBond-Breaker™ TCEP Solution, Neutral pH, 0.5 mL10% Pierce™ Trifluoroacetic Acid (TFA), Sequencing Grade, 0.5 mL1M MgCl2, 0.75 mL
Product Line: SureQuant™
Starting Material: Cells, Tissue
Workflow Step: Sample prep enrichment
For Use With (Application): MS-compatible immunoprecipitation
Description:
Protein A/G Magnetic Beads
For Use With (Equipment):
Mass spectrometer
Content And Storage:
Store trypsin at 4°C.Store trypsin storage solution at -20°C.
Detection Method:
Mass Spectrometry
Quantity:
20 Reactions
Kit Contents:
Trypsin Protease, 2 x 20 μgTrypsin Storage Solution, 250 μLProtein A/G Magnetic Beads, 1 mLIP-MS Cell Lysis Buffer, 100 mLIP-MS Wash Buffer A, 75 mLIP-MS Wash Buffer B, 40 mLIP Elution and MS Sample Prep Buffer, 10 mLAlkylation Solution, 1 mL/vialLow Protein-Binding Collection Tubes, 50 x 1.5 mLBond-Breaker™ TCEP Solution, Neutral pH, 0.5 mL10% Pierce™ Trifluoroacetic Acid (TFA), Sequencing Grade, 0.5 mL1M MgCl2, 0.75 mL
Product Line:
SureQuant™
Starting Material:
Cells, Tissue
Workflow Step:
Sample prep enrichment
For Use With (Application):
MS-compatible immunoprecipitation
Description: Streptavidin Magnetic Beads
For Use With (Equipment): Mass spectrometer
Content And Storage: Store trypsin at 4°C.Store trypsin storage solution at -20°C.
Detection Method: Mass Spectrometry
Quantity: 20 Reactions
Kit Contents: Trypsin Protease, 2 x 20 μgTrypsin Storage Solution, 250 μLStreptavidin Magnetic Beads, 1 mLIP-MS Cell Lysis Buffer, 100 mLIP-MS Wash Buffer A, 75 mLIP-MS Wash Buffer B, 40 mLIP Elution and MS Sample Prep Buffer, 10 mLAlkylation Solution, 1 mL/vialLow Protein-Binding Collection Tubes, 50 x 1.5 mLBond-Breaker™ TCEP Solution, Neutral pH, 0.5 mL10% Pierce™ Trifluoroacetic Acid (TFA), Sequencing Grade, 0.5 mL
Product Line: SureQuant™
Starting Material: Cells, Tissue
Workflow Step: Sample prep enrichment
For Use With (Application): MS-compatible immunoprecipitation
Description:
Streptavidin Magnetic Beads
For Use With (Equipment):
Mass spectrometer
Content And Storage:
Store trypsin at 4°C.Store trypsin storage solution at -20°C.
Detection Method:
Mass Spectrometry
Quantity:
20 Reactions
Kit Contents:
Trypsin Protease, 2 x 20 μgTrypsin Storage Solution, 250 μLStreptavidin Magnetic Beads, 1 mLIP-MS Cell Lysis Buffer, 100 mLIP-MS Wash Buffer A, 75 mLIP-MS Wash Buffer B, 40 mLIP Elution and MS Sample Prep Buffer, 10 mLAlkylation Solution, 1 mL/vialLow Protein-Binding Collection Tubes, 50 x 1.5 mLBond-Breaker™ TCEP Solution, Neutral pH, 0.5 mL10% Pierce™ Trifluoroacetic Acid (TFA), Sequencing Grade, 0.5 mL
Product Line:
SureQuant™
Starting Material:
Cells, Tissue
Workflow Step:
Sample prep enrichment
For Use With (Application):
MS-compatible immunoprecipitation
Description: SureQuant™ Multipathway Phosphopeptide Standard (100 fmol/μL)
For Use With (Equipment): __
Content And Storage: __
Detection Method: __
Quantity: 200 μL
Kit Contents: __
Product Line: __
Starting Material: __
Workflow Step: __
For Use With (Application): __
Description:
SureQuant™ Multipathway Phosphopeptide Standard (100 fmol/μL)
For Use With (Equipment):
__
Content And Storage:
__
Detection Method:
__
Quantity:
200 μL
Kit Contents:
__
Product Line:
__
Starting Material:
__
Workflow Step:
__
For Use With (Application):
__
Description: SureQuant™ Phosphopeptide Suitability Standard (500 fmol/μL)
For Use With (Equipment): __
Content And Storage: __
Detection Method: __
Quantity: 50 μL
Kit Contents: __
Product Line: __
Starting Material: __
Workflow Step: __
For Use With (Application): __
Description:
SureQuant™ Phosphopeptide Suitability Standard (500 fmol/μL)
For Use With (Equipment):
__
Content And Storage:
__
Detection Method:
__
Quantity:
50 μL
Kit Contents:
__
Product Line:
__
Starting Material:
__
Workflow Step:
__
For Use With (Application):
__