PIA45734
Thermo Scientific™ EasyPep™ MS Sample Prep Kits
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PIA45734-Each-of-1 | In Stock | ₹ 2,15,611.20 |
PIA45734 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 2,15,611.20
GST (18%): ₹ 38,810.016
Total Price: ₹ 2,54,421.216
Description
Maxi Sample Prep Kit
Product Type
Sample Preparation Kit
Content And Storage
Lysis Solution, 25 mL, store at 4°CUniversal Nuclease, 25 kU, store at -20°CReduction Solution, 7 mL, store at 4°CAlkylation Solution, 7 mL, store at 4°CEnzyme Reconstititution solution, 7 mL, store at 4°CPierce™ Trypsin/Lys-C Protease Mix, MS Grade, 8 x 100 μg, store at -20°CDigestion Stop Solution, 7 mL, store at 4°CPeptide Clean-Up Columns, 8 Ea., store at 4°CWash Solution A, 40 mL, store at 4°CWash Solution B, 3 x 27 mL, store at 4°CElution Solution, 2 x 20 mL, store at 4°CLow Protein Binding Collection Tubes, 2 mL, 40 Ea., store at 4°C
Shipping Condition
Wet Ice
Final Product Type
Peptides
Workflow Step
Protein Digestion
For Use With (Equipment)
Mass Spectrometer
Product Line
EasyPep™
Label or Dye
Unlabeled
Starting Material
Protein Samples
Detection Method
Mass Spectrometry
Quantity
8 Reactions
Related Products
Description
- EasyPep Mini MS Sample Prep Kits enable efficient and reproducible processing of cultured mammalian cells and tissues for proteomic MS analysis
- The kit contains pre-formulated buffers, MS-grade enzyme mix, peptide clean-up columns, and an optimized protocol to generate MS-compatible peptide samples in less than three hours
- Kits are optimized to process protein samples from 10–100 μg with a high yield of MS-ready peptides
- • Complete —includes pre-formulated reagents for lysis through digestion, peptide clean-up columns, and an optimized protocol for up to 20 samples • Optimized —streamlined protocol and reagents minimize the number of steps and time time needed to process samples • Flexible —reagents and protocol have been verified using cells, plasma, and tissue samples for 10 to 100 μg samples • Time-saving —sample processing time reduced from more than one day to less than three hours • Compatible —final preparation is ready for direct MS analysis and other downstream applications, including TMT labeling The sample preparation of peptides for MS analysis is complex, with numerous steps and varied protocols, resulting in sample variability in both quality and quantity
- This kit has been designed to improve reproducibility while saving hands-on and processing time
- The number of steps and processing time have been reduced through the addition of Universal Nuclease to reduce viscosity from nucleic acids (replacing sonication), a rapid 'one pot' reduction/alkylation solution for cysteine modification (carbamidomethylation, + 57.02), and a trypsin/Lys-C protease mix for more complete digestion
- In addition, the kit includes Peptide Clean-up columns and buffers to prepare detergent-free peptide samples for MS analysis
- The optimized reagents and protocol produce high-quality peptides that are compatible with TMT labeling and other downstream applications, or are ready for MS analysis.
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Description: Maxi Sample Prep Kit
Product Type: Sample Preparation Kit
Content And Storage: Lysis Solution, 25 mL, store at 4°CUniversal Nuclease, 25 kU, store at -20°CReduction Solution, 7 mL, store at 4°CAlkylation Solution, 7 mL, store at 4°CEnzyme Reconstititution solution, 7 mL, store at 4°CPierce™ Trypsin/Lys-C Protease Mix, MS Grade, 8 x 100 μg, store at -20°CDigestion Stop Solution, 7 mL, store at 4°CPeptide Clean-Up Columns, 8 Ea., store at 4°CWash Solution A, 40 mL, store at 4°CWash Solution B, 3 x 27 mL, store at 4°CElution Solution, 2 x 20 mL, store at 4°CLow Protein Binding Collection Tubes, 2 mL, 40 Ea., store at 4°C
Shipping Condition: Wet Ice
Final Product Type: Peptides
Workflow Step: Protein Digestion
For Use With (Equipment): Mass Spectrometer
Product Line: EasyPep™
Label or Dye: Unlabeled
Starting Material: Protein Samples
Detection Method: Mass Spectrometry
Quantity: 8 Reactions
Description:
Maxi Sample Prep Kit
Product Type:
Sample Preparation Kit
Content And Storage:
Lysis Solution, 25 mL, store at 4°CUniversal Nuclease, 25 kU, store at -20°CReduction Solution, 7 mL, store at 4°CAlkylation Solution, 7 mL, store at 4°CEnzyme Reconstititution solution, 7 mL, store at 4°CPierce™ Trypsin/Lys-C Protease Mix, MS Grade, 8 x 100 μg, store at -20°CDigestion Stop Solution, 7 mL, store at 4°CPeptide Clean-Up Columns, 8 Ea., store at 4°CWash Solution A, 40 mL, store at 4°CWash Solution B, 3 x 27 mL, store at 4°CElution Solution, 2 x 20 mL, store at 4°CLow Protein Binding Collection Tubes, 2 mL, 40 Ea., store at 4°C
Shipping Condition:
Wet Ice
Final Product Type:
Peptides
Workflow Step:
Protein Digestion
For Use With (Equipment):
Mass Spectrometer
Product Line:
EasyPep™
Label or Dye:
Unlabeled
Starting Material:
Protein Samples
Detection Method:
Mass Spectrometry
Quantity:
8 Reactions
Description: Lysis Buffer
Product Type: Lysis Buffer
Content And Storage: Store at 2°C to 8°C
Shipping Condition: Wet Ice
Final Product Type: Protein
Workflow Step: Cell Lysis
For Use With (Equipment): Mass Spectrometer
Product Line: EasyPep™
Label or Dye: Unlabeled
Starting Material: Protein Samples
Detection Method: Mass Spectrometry
Quantity: 100 mL
Description:
Lysis Buffer
Product Type:
Lysis Buffer
Content And Storage:
Store at 2°C to 8°C
Shipping Condition:
Wet Ice
Final Product Type:
Protein
Workflow Step:
Cell Lysis
For Use With (Equipment):
Mass Spectrometer
Product Line:
EasyPep™
Label or Dye:
Unlabeled
Starting Material:
Protein Samples
Detection Method:
Mass Spectrometry
Quantity:
100 mL
Description: __
Product Type: Substrate
Content And Storage: • 500 mL SuperSignal West Pico PLUS reagent, store at room temperature• 20 mL SuperSignal West Atto reagent, store at 2–8°C
Shipping Condition: Approved for shipment at Room Temperature or on Wet or Dry Ice
Final Product Type: __
Workflow Step: __
For Use With (Equipment): __
Product Line: SuperSignal™
Label or Dye: __
Starting Material: __
Detection Method: Chemiluminescence
Quantity: 1 Bundle
Description:
__
Product Type:
Substrate
Content And Storage:
• 500 mL SuperSignal West Pico PLUS reagent, store at room temperature• 20 mL SuperSignal West Atto reagent, store at 2–8°C
Shipping Condition:
Approved for shipment at Room Temperature or on Wet or Dry Ice
Final Product Type:
__
Workflow Step:
__
For Use With (Equipment):
__
Product Line:
SuperSignal™
Label or Dye:
__
Starting Material:
__
Detection Method:
Chemiluminescence
Quantity:
1 Bundle
Description: __
Product Type: Substrate
Content And Storage: • 100 mL SuperSignal West Femto reagent, store at 2–8°C• 20 mL SuperSignal West Atto reagent, store at 2–8°C
Shipping Condition: Approved for shipment at Room Temperature or on Wet or Dry Ice
Final Product Type: __
Workflow Step: __
For Use With (Equipment): __
Product Line: SuperSignal™
Label or Dye: __
Starting Material: __
Detection Method: Chemiluminescence
Quantity: 1 Bundle
Description:
__
Product Type:
Substrate
Content And Storage:
• 100 mL SuperSignal West Femto reagent, store at 2–8°C• 20 mL SuperSignal West Atto reagent, store at 2–8°C
Shipping Condition:
Approved for shipment at Room Temperature or on Wet or Dry Ice
Final Product Type:
__
Workflow Step:
__
For Use With (Equipment):
__
Product Line:
SuperSignal™
Label or Dye:
__
Starting Material:
__
Detection Method:
Chemiluminescence
Quantity:
1 Bundle