Thermo Scientific™ CaptureSelect™ Human Growth Hormone Affinity Matrix

Description

• CaptureSelect Human Growth Hormone (hGH) Affinity Matrix has been specifically designed for the purification of human growth hormone from recombinant sources
• Features of this affinity matrix include: Highly selective for recombinant hGH Efficient purification of recombinant hGH with high yields and retained activity Excellent scalability Non-animal-derived Recombinant hGH, also known as somatotropin or somatropin, is a peptide hormone that stimulates growth, cell reproduction, and regeneration in humans and other animals
• The high selectivity and yields obtained using CaptureSelect Human Growth Hormone Affinity Matrix enable a robust and efficient purification process with excellent purity obtained in one step
• Free of animal components CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology
• The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free)
• Main characteristics: Matrix: agarose-based, aldehyde-activated Average particle size: 65 ± 10 μm Ligand: CaptureSelect hGH affinity ligand Ligand coupling method: aldehyde coupling via NH 2 residues of the ligand Binding capacity: ∼3 mg/mL resin Elution conditions: 20 mM citric acid, pH 3.0 Flow characteristics: 50–200 cm/h (up to 2 bar) Formulation buffer: 20%(v/v) ethanol