Thermo Scientific™ CaptureSelect™ CH1-XL Affinity Matrix

Description

• CaptureSelect CH1-XL Affinity Matrix is the improved version of CaptureSelect IgG-CH1 Affinity Matrix
• The specificity is the same, but it features a wider elution range (pH 3.0-4.5), increased binding capacity, and improved stability at higher pH
• CaptureSelect CH1-XL Affinity Matrix recognizes the CH1 domain of human IgG antibodies, enabling purification of all subclasses (1, 2, 3, and 4) of human IgG and Fab fragments, independent of the light-chain isotype and source material
• CaptureSelect CH1-XL Affinity Matrix features include: Platform approach for purification of IgGs and their Fab fragments directly from complex source materials in a single step with high purity and yield Elution possible at pH 4.0–4.5 with 50 mM acetate buffer No co-purification of overproduced free light chain due to selectivity for the CH1 domain No binding to bovine antibodies, so presence of fetal bovine serum is not a problem Excellent scalability Non-animal derived Free of animal components CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology
• The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free)
• Main characteristics: Matrix: agarose-based, epoxide-activated Average particle size: 65 ± 10 μm Ligand: CaptureSelect CH1-XL affinity ligand Ligand coupling method: epoxide coupling Binding capacity: >15 mg/mL human Fab fragment depending on flow rate, column height, contact time
• For humanized Fabs lower capacities can be observed
• Elution conditions: 100 mM glycine buffer, pH 3.0; 50 mM acetate buffer, pH 4.0–4.5 Flow rate: 50–200 cm/h (up to 2 bar) Formulation buffer: 20% (v/v) ethanol