Thermo Scientific™ POROS™ CaptureSelect™ BacuClear Affinity Matrix

Description

• POROS CaptureSelect BacuClear Affinity Matrix is specifically designed for the removal of baculovirus contaminants when using baculovirus expression systems (BEVS) for the production of recombinant biomolecules
• It is particularly useful when expressing virus and virus-like particles (VLPs), which are similar in size and envelope structure to baculovirus, making discrimination and separation from the co-expressed baculovirus contaminants particularly challenging
• The baculovirus-specific CaptureSelect affinity ligand was developed to address this challenge and is coupled to the high-performing POROS matrix
• The large pore structure of the POROS beads enables efficient purification of larger biomolecules
• To remove baculovirus impurities, the affinity matrix is operated in flow-through mode
• As described by Moleirinho et
• al
• (1), successful removal of baculovirus particles from influenza VLPs, HCV VLPs, and AAVs was achieved with the affinity ligand identified (BV-2) coupled to the POROS matrix
• A dynamic binding capacity for baculovirus particles of 1.6 x 10 10 VG/mL affinity matrix was demonstrated with baculovirus removals observed above 70% and VLP recovery yields above 60%
• Features of this affinity matrix include: One-step removal of baculovirus virus particles from virus and VLP containing samples, in flow-through mode Enables a highly selective polishing step for baculovirus removal High specificity, capacity, and retained activity Excellent scalability Non-animal derived Free of animal components CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology
• The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free)
• Main characteristics: Matrix: cross-linked poly(styrene-divinylbenzene) Average particle size: 50 μm Ligand: POROS CaptureSelect BacuClear affinity ligand Ligand coupling method: epoxide Binding capacity: estimated >1 x 10 10 VG/mL of resin Regeneration conditions: acidic stripping condition with 0.5 M acetic acid, pH 2 Mechanical resistance: 100 bar (1450 psi, 10 MPa) Formulation buffer: 20% (v/v) ethanol 1
• M.G
• Moleirinho, R.P
• Fernandes, S.B
• Carvalho, S
• Bezemer, F
• Detmers, P Hermans, R.J.S
• Silva, P.M
• Alves, M.J.T
• Carrondo, C
• Peixoto
• Baculovirus affinity removal in viral-based bioprocesses
• Sep
• Purif
• Technol
• 241 (2020)