Cytiva His SpinTrap™ Spin Columns

Description

• Single-use spin columns prepacked with Ni Sepharose High Performance Medium that is precharged with nickel ions; negligible ion leakage One run in standard microcentrifuge takes approximatedly 10 min
• Yields up to 750μg purified protein per column Allows direct purification of both clarified and unclarifed cell lysates Also useful for screening large numbers of small-scale bacterial lysates Kit option contains buffer, allowing fast and convenient purifications without time-consuming buffer preparation step Medium Characteristics: Compatible with a wide range of reducing agents, detergents, denaturants and other additives Stable (long-term) in the pH range 3-12 Average particle size: 34μm Order Info Kit (28-9321-71) contains 50 columns and one pack of His Buffer Kit (2 x 100mL phosphate 8X stock solution, pH 7.4, and 100mL highly pure 2M imidazole, pH 7.4)