Cytiva His Mag Sepharose™ excel Chromatography Media

Description

• Samples that usually cause stripping of metal ions can therefore be added to the medium with retained protein-binding capacity and without pretreatment
• His Mag Sepharose excel is a magnetic IMAC medium precharged with nickel ions that are very strongly bound to a chelating ligand
• The medium enables an efficient and simplified workflow for capture, purification, and reproducible screening of histidine-tagged proteins
• Simplify small-scale purification and expression screening of histidine-tagged proteins secreted in eukaryotic cell culture
• Purify histidine-tagged proteins directly from samples that contain metal ion-stripping agents and save time and effort
• Increase target protein yield and decrease degradation through reduced and simplified sample handling
• Screen histidine-tagged proteins in parallel with high reproducibility and reliable results
• Capture histidine-tagged protein from different sample volumes; from low to high milliliter scale
• Simplify handling using visible and dense Sepharose-based magnetic beads
• Proteins are expressed and secreted into cell culture supernatants from eukaryotic cells such as insect cells or Chinese Hamster Ovary (CHO) cells
• The high density of the beads allows rapid capture by magnetic devices while the visibility of the beads ensures reliable collection of the bound histidine-tagged proteins during the purification procedure
• The base matrix is based on Sepharose medium with magnetite incorporated
• His Mag Sepharose is available as 10% medium slurry in three different pack sizes (2 x 1ml, 5 x 1ml, and 10 x 1ml) and can be used together with different test tubes and magnetic racks.