45-002-993
Cytiva HiPrep™ Sephacryl™ HR Prepacked Gel Filtration Columns
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 45-002-993-Each-of-1 | In Stock | ₹ 1,40,175.00 |
45-002-993 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,40,175.00
GST (18%): ₹ 25,231.50
Total Price: ₹ 1,65,406.50
Quantity
Each
Particle Size
25 to 75 μm
Bed Volume
320 mL
Diameter (Metric) Inner
2.6cm
Height (English)
23.62 in.
Material
Polypropylene
pH Range
3 to 11
Sample Type
Polysaccharides, macromolecules with extended structures and even small plasmids
Flow Rate
<0.5 mL/min.
For Use With (Equipment)
Low to medium pressure AKTA™ systems or pumped chromatography systems
Product Type
Sephacryl HR Pre-packed Gel Filtration Column
For Use With (Application)
Reproducible Protein purification, separating Polysaccharides, macromolecules with extended structures and small plasmids
Height (Metric)
60cm
Max. Pressure
1.5 bar [0.15 MPa] (22 psi)
Support Type
Allyl Dextran and N,N'-methylenebisacrylamide
Content And Storage
4°C to 30°C, 20% Ethanol
Related Products
Description
- Aids in preparative separation of biomolecules from up to 5mL (for 16mm column I.D.) and 13mL (for 26mm column I.D.)
- Provides maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability Prepacked columns for use with AKTAdesign™ or other pumped chromatography systems Available in two different prepacked column sizes - 16 x 600mm (120mL) and 26 x 60mm (320mL) Maximum pressure (over packed bed during operation) is 1.5 bar (22psi) Media Average particle size: 50μm Matrix: Allyl dextran and N, N'-methylenebisacrylamide pH Stability working range: 3 to 11 pH Stability cleaning range: 2 to 13 Chemical stability: all commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1% SDS, 20% ethanol, 30% propanol, 30% acetonitrile (tested at 40°C for one week), 0.5 M NaOH (only for cleaning-in-place) Pressure/Flow specification: >125cm/hr., 100kPa, XK 50/30, bed height 15cm Column Material (bed support): Polymethyl-Methacrylate HiPrep 16/60 Sephacryl S-400 HR Media: Fractionation [Mr] globular proteins: 2 x 104 -8 x 106 Exclusion limit [Mr] (globular proteins): ≈9 x 106 (9000kDa) Fractionation range [Mr] DNA exclusion limit: 271bp Fractionation [Mr] Dextrans: 1 x 104 -2 x 106 HiPrep 16/60 Sephacryl S-500 HR Media: Exclusion limit for Dextrans: 100 x 106 Fractionation range [Mr] DNA exclusion limit: 1078bp Fractionation [Mr] Dextrans: 4 x 104 -2 x 107
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Quantity: Each
Particle Size: 25 to 75 μm
Bed Volume: 320 mL
Diameter (Metric) Inner: 2.6cm
Height (English): 23.62 in.
Material: Polypropylene
pH Range: 3 to 11
Sample Type: Polysaccharides, macromolecules with extended structures and even small plasmids
Flow Rate: <0.5 mL/min.
For Use With (Equipment): Low to medium pressure AKTA™ systems or pumped chromatography systems
Product Type: Sephacryl HR Pre-packed Gel Filtration Column
For Use With (Application): Reproducible Protein purification, separating Polysaccharides, macromolecules with extended structures and small plasmids
Height (Metric): 60cm
Max. Pressure: 1.5 bar [0.15 MPa] (22 psi)
Support Type: Allyl Dextran and N,N'-methylenebisacrylamide
Content And Storage: 4°C to 30°C, 20% Ethanol
Quantity:
Each
Particle Size:
25 to 75 μm
Bed Volume:
320 mL
Diameter (Metric) Inner:
2.6cm
Height (English):
23.62 in.
Material:
Polypropylene
pH Range:
3 to 11
Sample Type:
Polysaccharides, macromolecules with extended structures and even small plasmids
Flow Rate:
<0.5 mL/min.
For Use With (Equipment):
Low to medium pressure AKTA™ systems or pumped chromatography systems
Product Type:
Sephacryl HR Pre-packed Gel Filtration Column
For Use With (Application):
Reproducible Protein purification, separating Polysaccharides, macromolecules with extended structures and small plasmids
Height (Metric):
60cm
Max. Pressure:
1.5 bar [0.15 MPa] (22 psi)
Support Type:
Allyl Dextran and N,N'-methylenebisacrylamide
Content And Storage:
4°C to 30°C, 20% Ethanol
Quantity: 25 mL
Particle Size: 75 μm
Bed Volume: __
Diameter (Metric) Inner: __
Height (English): __
Material: __
pH Range: 2 to 13
Sample Type: __
Flow Rate: 600 cm/Hoμr
For Use With (Equipment): __
Product Type: Capto Blue Media
For Use With (Application): For Enzyme PurifiCatIon, Capture of Albumin
Height (Metric): __
Max. Pressure: 300 kPa
Support Type: __
Content And Storage: __
Quantity:
25 mL
Particle Size:
75 μm
Bed Volume:
__
Diameter (Metric) Inner:
__
Height (English):
__
Material:
__
pH Range:
2 to 13
Sample Type:
__
Flow Rate:
600 cm/Hoμr
For Use With (Equipment):
__
Product Type:
Capto Blue Media
For Use With (Application):
For Enzyme PurifiCatIon, Capture of Albumin
Height (Metric):
__
Max. Pressure:
300 kPa
Support Type:
__
Content And Storage:
__
Quantity: __
Particle Size: __
Bed Volume: __
Diameter (Metric) Inner: __
Height (English): __
Material: __
pH Range: __
Sample Type: __
Flow Rate: __
For Use With (Equipment): __
Product Type: illustra TempliPhi 2000 Reaction Kit
For Use With (Application): High-throughput preparation of circular DNA templates for cycle sequencing, cloning, and transformation.
Height (Metric): __
Max. Pressure: __
Support Type: __
Content And Storage: Store Denature buffer and control DNA at -70°C or -15°C to -30°C. Store TempliPhi premix at -70°C.Do not store TempliPhi premix at -15°C to -30°C. Thaw the premix on ice and maintain at 0–4°C during handling. Do not allow premix to warm above 4°C prior
Quantity:
__
Particle Size:
__
Bed Volume:
__
Diameter (Metric) Inner:
__
Height (English):
__
Material:
__
pH Range:
__
Sample Type:
__
Flow Rate:
__
For Use With (Equipment):
__
Product Type:
illustra TempliPhi 2000 Reaction Kit
For Use With (Application):
High-throughput preparation of circular DNA templates for cycle sequencing, cloning, and transformation.
Height (Metric):
__
Max. Pressure:
__
Support Type:
__
Content And Storage:
Store Denature buffer and control DNA at -70°C or -15°C to -30°C. Store TempliPhi premix at -70°C.Do not store TempliPhi premix at -15°C to -30°C. Thaw the premix on ice and maintain at 0–4°C during handling. Do not allow premix to warm above 4°C prior
Quantity: 100 mL
Particle Size: 90μm
Bed Volume: __
Diameter (Metric) Inner: __
Height (English): __
Material: __
pH Range: 2 to 12
Sample Type: __
Flow Rate: 150 to 600cm/hr.
For Use With (Equipment): __
Product Type: Ni Sepharose excel chromato graphy media
For Use With (Application): Capture and purification of His-tagged Proteins secreted into eukaryotic cell culture supernatants by immobilized Metal ion affinity chromato graphy (IMAC)
Height (Metric): __
Max. Pressure: __
Support Type: 6% Highly Cross-linked Agarose
Content And Storage: 4° to 30°C
Quantity:
100 mL
Particle Size:
90μm
Bed Volume:
__
Diameter (Metric) Inner:
__
Height (English):
__
Material:
__
pH Range:
2 to 12
Sample Type:
__
Flow Rate:
150 to 600cm/hr.
For Use With (Equipment):
__
Product Type:
Ni Sepharose excel chromato graphy media
For Use With (Application):
Capture and purification of His-tagged Proteins secreted into eukaryotic cell culture supernatants by immobilized Metal ion affinity chromato graphy (IMAC)
Height (Metric):
__
Max. Pressure:
__
Support Type:
6% Highly Cross-linked Agarose
Content And Storage:
4° to 30°C