Cytiva HiPrep™ Sephacryl™ HR Prepacked Gel Filtration Columns

Description

• Aids in preparative separation of biomolecules from up to 5mL (for 16mm column I.D.) and 13mL (for 26mm column I.D.)
• Provides maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability Prepacked columns for use with AKTAdesign™ or other pumped chromatography systems Available in two different prepacked column sizes - 16 x 600mm (120mL) and 26 x 60mm (320mL) Maximum pressure (over packed bed during operation) is 1.5 bar (22psi) Media Average particle size: 50μm Matrix: Allyl dextran and N, N'-methylenebisacrylamide pH Stability working range: 3 to 11 pH Stability cleaning range: 2 to 13 Chemical stability: all commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1% SDS, 20% ethanol, 30% propanol, 30% acetonitrile (tested at 40°C for one week), 0.5 M NaOH (only for cleaning-in-place) Pressure/Flow specification: >125cm/hr., 100kPa, XK 50/30, bed height 15cm Column Material (bed support): Polymethyl-Methacrylate HiPrep 16/60 Sephacryl S-400 HR Media: Fractionation [Mr] globular proteins: 2 x 104 -8 x 106 Exclusion limit [Mr] (globular proteins): ≈9 x 106 (9000kDa) Fractionation range [Mr] DNA exclusion limit:    271bp Fractionation [Mr] Dextrans: 1 x 104 -2 x 106 HiPrep 16/60 Sephacryl S-500 HR Media: Exclusion limit for Dextrans: 100 x 106 Fractionation range [Mr] DNA exclusion limit: 1078bp Fractionation [Mr] Dextrans: 4 x 104 -2 x 107