45-003-011
Cytiva Ni Sepharose™ excel
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 45-003-011-Each-of-1 | In Stock | ₹ 2,38,075.00 |
45-003-011 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 2,38,075.00
GST (18%): ₹ 42,853.50
Total Price: ₹ 2,80,928.50
Buffer
0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol
Quantity
100 mL
pH Range
2 to 12
Support Type
6% Highly Cross-linked Agarose
For Use With (Application)
Capture and purification of His-tagged Proteins secreted into eukaryotic cell culture supernatants by immobilized Metal ion affinity chromato graphy (IMAC)
Product Type
Ni Sepharose excel chromato graphy media
Flow Rate
150 to 600cm/hr.
Particle Size
90μm
Content And Storage
4° to 30°C
Matrix
Ni-Sepharose
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Description
- Ni Sepharose excel enables direct loading of samples without having to perform extensive and time-consuming pretreatment procedures
- Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity
- Increase target protein yield and decrease degradation through reduced and simplified sample handling
- Choose between several formats for screening and preparative purification of histidine-tagged proteins
- Ni Sepharose excel is an IMAC medium precharged with nickel ions very strongly bound to a chelating ligand, allowing loading of samples that usually cause stripping of metal ions
- Ni Sepharose excel is designed primarily for capture and purification of histidine-tagged proteins secreted into cell culture supernatants from eukaryotic cells such as insect cells or CHO cells.
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Buffer: 0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol
Quantity: 100 mL
pH Range: 2 to 12
Support Type: 6% Highly Cross-linked Agarose
For Use With (Application): Capture and purification of His-tagged Proteins secreted into eukaryotic cell culture supernatants by immobilized Metal ion affinity chromato graphy (IMAC)
Product Type: Ni Sepharose excel chromato graphy media
Flow Rate: 150 to 600cm/hr.
Particle Size: 90μm
Content And Storage: 4° to 30°C
Matrix: Ni-Sepharose
Buffer:
0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol
Quantity:
100 mL
pH Range:
2 to 12
Support Type:
6% Highly Cross-linked Agarose
For Use With (Application):
Capture and purification of His-tagged Proteins secreted into eukaryotic cell culture supernatants by immobilized Metal ion affinity chromato graphy (IMAC)
Product Type:
Ni Sepharose excel chromato graphy media
Flow Rate:
150 to 600cm/hr.
Particle Size:
90μm
Content And Storage:
4° to 30°C
Matrix:
Ni-Sepharose
Buffer: 0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol
Quantity: 500 mL
pH Range: 2 to 12
Support Type: 6% Highly Cross-linked Agarose
For Use With (Application): Capture and purification of His-tagged Proteins secreted into eukaryotic cell culture supernatants by immobilized Metal ion affinity chromato graphy (IMAC)
Product Type: Ni Sepharose excel chromato graphy media
Flow Rate: 150 to 600cm/hr.
Particle Size: 90μm
Content And Storage: 4° to 30°C
Matrix: Ni-Sepharose
Buffer:
0.01M HCl and 0.01M NaOH (one week), 10mM EDTA, 5mM DTT, 1M NaOH, 5mM TCEP, 20mM beta-Mercapto ethanol and 6M Guanidine-HCl (24 Hours), 500mM Imidazole and 100mM EDTA (2 Hours), 30% 2-Propanol
Quantity:
500 mL
pH Range:
2 to 12
Support Type:
6% Highly Cross-linked Agarose
For Use With (Application):
Capture and purification of His-tagged Proteins secreted into eukaryotic cell culture supernatants by immobilized Metal ion affinity chromato graphy (IMAC)
Product Type:
Ni Sepharose excel chromato graphy media
Flow Rate:
150 to 600cm/hr.
Particle Size:
90μm
Content And Storage:
4° to 30°C
Matrix:
Ni-Sepharose
Buffer: __
Quantity: 5 x 1 mL
pH Range: 2 to 12
Support Type: 6% Highly Cross-linked Agarose
For Use With (Application): __
Product Type: __
Flow Rate: 1 to 4 mL/min.
Particle Size: __
Content And Storage: 4°C to 30°C
Matrix: __
Buffer:
__
Quantity:
5 x 1 mL
pH Range:
2 to 12
Support Type:
6% Highly Cross-linked Agarose
For Use With (Application):
__
Product Type:
__
Flow Rate:
1 to 4 mL/min.
Particle Size:
__
Content And Storage:
4°C to 30°C
Matrix:
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Buffer: __
Quantity: 5 x 5 mL
pH Range: 2 to 12
Support Type: 6% Highly Cross-linked Agarose
For Use With (Application): __
Product Type: __
Flow Rate: 5 to 20 mL/min.
Particle Size: __
Content And Storage: 4°C to 30°C
Matrix: __
Buffer:
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Quantity:
5 x 5 mL
pH Range:
2 to 12
Support Type:
6% Highly Cross-linked Agarose
For Use With (Application):
__
Product Type:
__
Flow Rate:
5 to 20 mL/min.
Particle Size:
__
Content And Storage:
4°C to 30°C
Matrix:
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