MilliporeSigma™ Calbiochem™ SKP2 E3 Ligase Inhibitor II, SMIP004

Description

• A cell-permeable phenylacetamide compound that is shown to upregulate p21 Cip1/Waf1 and/or p27 Kip1 in prostate cancer LNCaP, PC3, DU145, but not HeLa, cultures (40μM for 24 h) with a concomitant downregulation of the F-box E3 ubiquitin ligase Skp2 and a delayed G1-to-S cell cycle transition, displaying no apparent effect toward cell cycle progression or p21 and p27 levels in non-cancer human fibroblast IMR90
• SMIP004 antiproliferative activity against LNCaP, PC3, DU145 soft agar colony formation is mainly cytostatic with cytotoxicity observed only in LNCaP cultures at high concentrations (IC 50 = 40μM in 72 h), but not PC3 or DU145 even at concentrations as high as 80μM and for up to 96 h
• Please note that the molecular weight for this compound is batch-specific due to variable water content
• Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight
• The molecular weight provided represents the baseline molecular weight without water.