MilliporeSigma™ Calbiochem™ iPSC Induction Enhancer II, OAC1

Description

• A cell-permeable pyrrolopyridinyl-benzamide compound that is reported to enhance the efficiency of 4F/4TF-induced iPSC generation from MEFs cultured in either feeder cell-containing ESC media or in feeder-/serum-free iSF1 media (1μM OAC1 treatment starts 48 h after viral transduction)
• OCA1 is shown to increase cellular Oct4, Nanog, Sox2, and Tet1 mRNA levels via a yet unidentified mechanism, although OAC1 is reported not affect Oct4 promoter methylation, cellular Wnt signaling, or p53 and p21 mRNA levels
• Six other iPSC reprogramming improving reagents, AM580, AzaC, BIX-01294 (Cat
• No
• 382190), Tranylcypromine (Cat
• No
• 616431), VPA (Cat
• No
• 676380), and Vitamin C (Cat
• No
• 1813), in comparison, do not exhibit Oct4 and Nanog promoters enhancing activity, while BIO (Cat
• No
• 361550) is demonstrated to be 3.8- and 6-fold less potent than OAC1, respectively, in inducing Oct4 and Nanog transcription.