MilliporeSigma™ Calbiochem™ Novel Inducer of Death-1, NID-1

Description

• A cell-permeable, nitrothiophenylpropenamide compound that induces active, energy-dependent, and rapid non-apoptotic cell death (EC 50 = 1.65μM) in several transformed (DRD, RKO, H1299) and non-transformed (BJeH ) human cells, in a variety of cancer cell lines, and in mouse embryonic fibroblasts
• The cell death appears to be non-necrotic, non-oxidative and is independent of DNA damage or cell proliferation
• NID-1 induced cell death, although distinct from macroautophagy, does show PI 3-kinase suppression, ATG5-dependence, p62 degradation, and higher LC3 levels
• Also, NID-1-induced death is independent of caspases, Bax and Bak and requires lysosomal activity and cytosolic vacuolization
• Rescues mutant-huntingtin-induced cell death in a cell-based model of Huntington’s disease
• Shown to increase cathepsin L levels, but does not affect cathepsins B, C, and H.