Thermo Scientific™ CaptureSelect™ C-tagXL MiniChrom™ Pre-packed Column

Description

• CaptureSelect C-tagXL MiniChrom™ columns are designed for bench-scale resin screening, process development, and sample preparation
• These pre-packed columns are ready to connect and have the following features: Dimensions of 1 mL (0.8 x 2 cm) and 5 mL (0.8 x 10 cm) Direct connection to standard chromatography systems Packed with CaptureSelect C-tagXL Affinity Matrix, offering high dynamic binding capacity and high resolution over a wide range of flow rates The 1-mL columns can be used for quick screening of application feasibility and lab-scale purification on a convenient and easy-to-use pre-packed column
• The 10-cm bed height of the 5-mL column allows initial process development on a bench scale
• CaptureSelect C-tagXL Affinity Matrix has a unique selectivity for a small 4-amino acid peptide tag (E-P-E-A, glutamic acid-proline-glutamic acid-alanine)
• CaptureSelect C-tagXL Affinity Matrix purifies the C-terminal tagged proteins with high affinity and selectivity, even in the presence of urea and guanidine HCl, from complex mixtures like cell culture harvests and periplasmic fractions in a one-step process
• Mild elution conditions at neutral pH can be applied using magnesium chloride or propylene glycol, which ensures high-activity recoveries of pH-sensitive target proteins
• The affinity resin recognizes the E-P-E-A tag sequence when fused either directly to the C-terminus of a protein or through a linker between the C-terminus and the E-P-E-A tag
• Features of this affinity matrix include: Mild elution, making it suitable for pH-sensitive proteins Binding of the tagged proteins under denaturing conditions (like dissolved inclusion bodies) Excellent scalability Non-animal-derived Free of animal components CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology
• The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free)
• Main characteristics: Matrix: agarose-based, epoxide-activated Average particle size: 65 ± 10 μm Ligand: CaptureSelect C-tagXL affinity ligand Ligand coupling method: epoxide coupling Binding capacity: 400 nmol/mL resin depending on flow rate, column height, contact time Elution conditions: Acidic: 20 mM citric acid or acetic acid, pH 3–4; 100 mM glycine buffer, pH 2–3
• Neutral (pH 7.0–7.4): 20 mM Tris, 2.0 M MgCl 2 ; 20 mM Tris, 1.0 M NaCl, 50% (v/v) propylene glycol; 20 mM Tris, 2 mM 'S-E-P-E-A' peptide Column dimensions: 1 mL (0.8 x 2 cm) and 5 mL (0.8 x 10 cm) Flow characteristics: 75–150 cm/h (up to 2 bar) Formulation buffer: 20%(v/v) ethanol