BD anti-CD8 Magnetic Particles, Clone: G28

Description

• BD IMag™ anti-rat CD8 Magnetic Particles - DM are magnetic nanoparticles that have monoclonal antibody conjugated to their surfaces
• These particles are optimized for the positive selection or depletion of CD8-bearing T lymphocytes using the BD IMagnet™
• The G28 antibody reacts with the V-like domain of the α chain of the CD8 differentiation antigen
• CD8α is an approximately 32-34kDa cell surface receptor expressed either as a heterodimer with the CD8 β chain (CD8 αβ) or as a homodimer (CD8 αα)
• The CD8 αβ heterodimer (CD8a and CD8b, respectively) is located on the surface of most thymocytes and a subpopulation of mature T lymphocytes (ie, MHC class I-restricted T cells, including most T suppressor/cytotoxic cells)
• Intestinal intraepithelial lymphocytes, many CD8+ T cells of athymic rats, many activated CD4+ T cells, and most NK cells express CD8a without CD8b
• It has been suggested that the expression of the CD8a/CD8b heterodimer is restricted to thymus-derived T lymphocytes
• CD8a reportedly is not expressed on resting CD4+ T-helper cells
• A single-cell suspension from the lymphoid tissue of interest is labeled with BD IMag™ anti-rat CD8 Magnetic Particles - DM according to the following Magnetic Labeling Protocol
• This labeled cell suspension is then placed within the magnetic field of the BD IMagnet™ (Mfr
• No
• 552311)
• Labeled cells migrate toward the magnet (positive fraction), leaving the unlabeled cells in suspension so they can be drawn off (negative fraction)
• The tube is then removed from the magnetic field for resuspension of the positive fraction
• The separation is repeated twice to increase the purity of the positive fraction
• The magnetic separation steps are diagrammed in the Separation Flow Chart
• After the positive fraction is washed, the small size of the magnetic particles allows the positive fraction to be further evaluated in downstream applications such as flow cytometry
• Cell Separation