Thermo Fisher Scientific DDM/CHS Solution (10:1)

Description

• A ready-to-use solution for solubilization of membrane proteins while maintaining structural integrity and activity The stable pre-formulated solution contains a mixture of dodecyl-β-D-maltoside (DDM) (100 mg/mL) and cholesteryl hemisuccinate (CHS) (10 mg/mL) in deionized water with final concentrations of 10% and 1%, respectively Typical working concentrations are 1% DDM/0.1% CHS for protein extraction and 0.1% DDM/0.01% CHS for protein purification Features of DDM/CHS Solution include: Ready-to-use liquid formulation Mixture of nonionic detergent, DDM, with dual hydrophobic/hydrophilic properties and anionic detergent, CHS, that aids in the stabilization of large unilamellar vesicles DDM/CHS micelles aid in solubilization of membrane proteins while preserving structure and function Suitable for protein solubilization and stabilization for single particle cryo-EM analysis Properties of DDM: Molecular formula: C 24 H 46 O 11 Molecular weight: 510.6 g/mol Detergent class: Non-ionic Micelle molecular weight: 50,000 g Critical micelle concentration (CMC): 0.17 mM (0.009% w/v) in water; 0.12 mM (0.006% w/v) in 0.2 M NaCl Properties of CHS: Molecular formula: C 31 H 50 O 4 · C 4 H 11 NO 3 Molecular weight: 607.9 g/mol Detergent: Ionizable anionic Function: Stabilizes large unilamellar vesicles