Description

• Cyclohexyl maltoside detergents are a class of specialized non-ionic detergents designed for the solubilization, stabilization, and manipulation of biomolecules, particularly membrane proteins
• They provide efficient solubilization of membrane proteins from lipid bilayers, allowing for their extraction and study in solution
• Cyclohexyl maltoside detergents are widely used in protein purification, structural biology studies, and other research involving membrane proteins
• They can be employed in various experimental techniques, including crystallization, spectroscopy, and functional analysis
• Features of cyclohexyl maltoside detergents • Gentle—preserves the native conformation and activity of membrane proteins • Enhanced stability during extraction and downstream applications • High-purity with low UV absorptivity Overall, cyclohexyl maltoside detergents are versatile and can be used in various experimental techniques, including protein purification, crystallization, spectroscopy, and structural biology studies
• They are compatible with a wide range of biomolecules and can be employed for both soluble and membrane protein research
• 5-Cyclohexyl-1-Pentyl-β-D-Maltoside 5-Cyclohexyl-1-Pentyl-β-D-Maltoside (Cymal-5) has a specific combination of a cyclohexyl and pentyl alkyl chain attached to the maltose moiety
• This combination of alkyl chains can enhance the detergent's hydrophobicity, solubilization properties, and interactions with certain types of membrane proteins
• Cymal-5 is often utilized for solubilization and stabilization of membrane protein complexes
• The solubilization efficiency allows for the extraction of intact complexes, helping to preserve their native structure and functionality
• It is important to note that the specific solubilization efficiency of Cymal-5 can vary depending on the specific protein, its structural features, and the experimental conditions
• Properties of Cymal-5 • CMC: (H 2 O) ~ 2.4-5.0 mM (0.12%) • Aggregation number: (H 2 O) ~ 47 • Purity: ≥ 99% β+α (by HPLC analysis) • pH: 5-8 (1% solution in water) 6-Cyclohexyl-1-Hexyl-β-D-Maltoside 6-Cyclohexyl-1-Hexyl-β-D-Maltoside (Cymal-6) has a hexyl (C6) alkyl chain attached to the maltose moiety
• The longer hexyl alkyl chain in Cymal-6 provides it with greater hydrophobicity compared to other cyclohexyl maltoside detergents
• Due to its enhanced hydrophobicity, Cymal-6 may be particularly effective in solubilizing membrane proteins that have higher hydrophobic regions or multiple transmembrane domains that often require stronger detergent interactions
• The longer alkyl chain may also lead to the formation of larger micelles
• Cymal-6  is well-suited for structural biology studies, such as protein crystallization or nuclear magnetic resonance (NMR) spectroscopy
• Properties of Cymal-6 • CMC: (H 2 O) ~ 0.56 mM (0.028%) • Aggregation number: (H 2 O) ~ 91 • Purity: ≥ 99% β+α (by HPLC analysis) • pH: 5-8 (1% solution in water) 7-Cyclohexyl-1-Heptyl-β-D-Maltoside 7-Cyclohexyl-1-Heptyl-β-D-Maltoside (Cymal-7) has a specific combination of a cyclohexyl and heptyl alkyl chain attached to the maltose moiety
• The presence of both cyclohexyl and heptyl alkyl chains in Cymal-7 contributes to its hydrophobicity
• Its specific alkyl chain combination can contribute to improved stability, helping to maintain the structural integrity and functionality of proteins during solubilization and downstream applications
• The longer alkyl chain may also lead to the formation of larger micelles
• Cymal-7 is well-suited for structural biology studies, such as protein crystallization or nuclear magnetic resonance (NMR) spectroscopy
• Properties of Cymal-7 • CMC: (H 2 O) ~ 0.19 mM (0.0099%) • Aggregation number: (H 2 O) ~ 150 • Purity: ≥ 99% β+α (by HPLC analysis) • pH: 5-8 (1% solution in water)