Thermo Scientific™ Pierce™ Anti-c-Myc Agarose

Description

• Purify recombinant c-Myc-tagged proteins expressed in human in vitro expression systems and bacterial and mammalian cell lysates with Thermo Scientific™ Pierce™ Anti-c-Myc Agarose, a high-affinity immunoprecipitation resin
• Thermo Scientific Pierce Anti-c-Myc Agarose is a high-affinity immunoprecipitation resin ideal for purification of recombinant c-Myc-tagged proteins expressed in human in vitro expression systems and bacterial and mammalian cell lysates
• The anti-c-Myc antibody used to manufacture Pierce Anti-c-Myc Agarose is a highly specific mouse IgG1 monoclonal antibody (clone 9E10) that recognizes the c-Myc epitope tag (EQKLISEEDL) derived from the human c-myc oncogene (p62 c-myc)
• The support is crosslinked 4% beaded agarose
• This anti-c-Myc affinity resin can be packed into gravity purification columns, spin purification columns or cartridges for FPLC instruments to purify c-Myc-fusion proteins expressed in bacterial or mammalian cells
• Highlights: Specific – highly specific anti-c-Myc monoclonal antibody (clone 9E10) enables high yield and high purity for immunoprecipitation Scalable – available in 2 and 10mL resin package sizes to allow for larger scale purifications or immunoprecipitations Versatile – can be used in spin or gravity columns as well as in FPLC cartridges Convenient – reagents to elute and detect c-Myc tagged fusion proteins are available separately Applications: Purification of c-Myc fusion proteins expressed in the Pierce Human In Vitro Translation Kits Large scale purification of recombinant c-Myc tagged proteins High-throughput enrichment of fusion proteins and interacting partners IP and co-IP experiments (as with the Pierce c-Myc-Tag IP/co-IP Kit) Product Details: Pierce Anti-c-Myc Agarose consists of a highly specific monoclonal anti-c-Myc antibody that is covalently immobilized on a crosslinked 4% beaded agarose support
• The product is supplied as a 25% slurry
• Upon incubation with a sample, c-Myc tagged fusion protein is captured on the agarose beads
• After simple washing steps, the tagged protein is easily eluted from the resin using 0.1M glycine (pH 2.0-2.8), 3M NaSCN or 50mM NaOH depending on the downstream application of the purified protein
• For elution of highly functional proteins, Pierce c-Myc-peptide can also be used to elute the c-Myc tagged protein
• Anti-c-Myc antibody may be used to detect the presence of the tagged protein by Western blot
• In experiments with a c-Myc-tagged fusion protein (26 to 29kDa), the resin provided a binding capacity of 102 to 144nmol protein per mL of settled resin
• The elution capacity was at least 18 to 19nmol per mL of settled resin using 0.1M glycine, pH 2.8
• Binding and elution capacity will vary depending on the c-Myc-fusion protein and the method of elution.