Thermo Scientific™ Pierce™ Avidin Agarose

Description

• Thermo Scientific™ Pierce Avidin Agarose is high-quality beaded agarose resin for biotin affinity procedures
• Additional Information and Online Ordering This avidin affinity resin is crosslinked beaded agarose onto which purified avidin has been covalently immobilized
• The biotin-binding agarose beads are available as pre-packed columns or resin slurry
• The resin is designed for use in a variety of applications for the affinity purification of biotinylated macromolecules
• Although streptavidin provides superior performance generally, avidin is more effective in certain situations
• Avidin is a tetrameric glycoprotein rich in tryptophan that is present in chicken egg white and the tissues of birds, and avidin is highly resistant to denaturation by acids or proteolytic enzymes
• Avidin Agarose Resin consists of purified avidin that has been immobilized to crosslinked 6% beaded agarose
• The resulting affinity support is leak-resistant, stable at pH from 2 to 11, and ideal for gravity-flow and low-speed centrifugation applications
• Highlights: Immobilized ligand is purified avidin from chicken egg whites Support is crosslinked 6% beaded agarose resin (agarose beads) Supplied as a stable aqueous slurry or pre-packed columns Binds free biotin and biotinylated molecules with high affinity Recommended for: Binding biotinylated anti-transferrin from serum; Binding biotinyl peptides and elution with an SDS/urea solution; Hybridization of biotinylated RNA to its complementary DNA and binding to immobilized avidin, with subsequent elution of the single-stranded DNA; Purification of double-stranded DNA