Thermo Scientific™ Pierce™ Iminobiotin Agarose

Description

• Thermo Scientific™ Pierce Iminobiotin and Biotin Resins provide for unusual affinity purification experiments involving clean-up or removal of streptavidin or avidin protein components
• Pierce Biotin Agarose and Iminobiotin Agarose are beaded agarose supports onto which biotin and iminobiotin moieties have been covalently immobilized
• Typical avidin-biotin affinity procedures use immobilized avidin or streptavidin proteins to capture biotin-tagged molecules from a sample
• Occasionally, however, the reverse situation arises in which immobilized biotin in needed to capture avidin or streptavidin components from a sample
• These biotin and iminobiotin resins address this special situation
• Immobilized biotin binds avidin and streptavidin very strongly, making it appropriate for non-reversible binding applications
• Immobilized iminobiotin is the guanido analog of biotin
• The dissociation constant of the avidin-iminobiotin complex is pH dependent
• At pH 9.5 to 11, the avidin-iminobiotin complex will bind tightly
• At pH 4, the avidin-iminobiotin complex will dissociate
• Thus, iminobiotin agarose can be used for situations requiring reversible binding and elution of avidin components
• Highlights: Enable purification or removal of avidin or streptavidin samples Resins are crosslinked beaded agarose onto which biotin or iminobiotin are covalently immobilized Use Biotin Agarose for non-reversible binding (e.g., removal of avidin components from a sample) Use Iminobiotin Agarose for reversible binding (e.g., non-denaturing purification of avidin)