Thermo Scientific™ MagnaBind™ Protein G Beads

Description

• Thermo Scientific™ MagnaBind Magnetic Beads are available with streptavidin, Protein A, Protein G, GAM IgG, GAR IgG and other ligands for use in small-scale benchtop magnetic affinity protocols
• MagnaBind Magnetic Beads enable small-scale affinity purification and cell-separation without columns or centrifugation
• The beads are silanized iron oxide magnetic particles activated with various affinity ligands
• After a binding step in an affinity purification procedure, the magnetic particles are easily and rapidly collected by placing the microcentrifuge tube or reaction vessel next to an appropriate rare-earth magnet
• MagnaBind Beads respond rapidly to MagnaBind Magnets
• The particles can be easily dispersed and regathered multiple times (i.e., they will not irreversibly aggregate) because they do not have any magnetic memory
• MagnaBind Beads are available pre-coated with Protein A, Protein G, streptavidin, anti-mouse or anti-rabbit antibodies
• Also available are beads activated with either amine or carboxyl groups as targets for customized covalent immobilization other proteins or ligands
• Highlights: Available pre-coated with popular affinity ligands or derivatized for covalent attachment of proteins and other specific ligands Beads do not irreversibly aggregate because they have no magnetic memory; collect and disperse the beads multiple times if needed Most separations require a short five- to 10-minute benchtop procedure Properties of underivatized MagnaBind Beads: Composition: silanized iron oxide Magnetization: 25 to 35 EMU/g Type of Magnetization: superparamagnetic (no magnetic memory) Surface Area: greater than 100m 2 /g Bead Size: 1 to 4μm diameter Settling Rate: 4% in 30 minutes Effective Density: 2.5g/mL Number of Beads: 1 x 10 8 beads per milliliter pH Stability: aqueous solution, above pH 4.0 Concentration: 5mg/mL Requires: To remove MagnaBind Beads from suspension, an external magnetic field is used; see the ordering table for a selection of magnets
• Recommended for: Cell sorting using either positive or negative selection; Protein purification or immunoassays using either direct or indirect methods; Immunoprecipiation